白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析

Effect of Preliminary Immune Activity and Structural Identification of a Polysaccharide Extracted from Oldenlandia diffusa

摘要:

目的: 分离纯化白花蛇舌草总多糖,研究其对环磷酰胺致免疫低下小鼠的免疫增强活性.方法: 采用超滤技术分离纯化白花蛇舌草总多糖,高效凝胶渗透色谱-示差检测器方法测定相对分子质量,柱前衍生化HPLC分析其单糖组成;采用环磷酰胺致免疫低下小鼠碳粒廓清试验研究其免疫活性.结果: 从白花蛇舌草中分离得到1个单一多糖组分ODP-1,其相对分子质量20.88 kDa,单糖组成为甘露糖-鼠李糖-半乳糖醛酸-葡萄糖-半乳糖-阿拉伯糖,摩尔比0.005:0.033:0.575:1.000:0.144:0.143;免疫活性试验结果表明,ODP-1可升高环磷酰胺致免疫低下小鼠廓清指数、吞噬指数、胸腺指数和脾指数.结论: 白花蛇舌草多糖ODP-1为均一多糖,具有免疫增强活性.

Abstract:

Objective:The polysaccharide was separated and purified from Oldenlandia diffusa and the immuno-enhancing activity was evaluated in immunosuppression mice by intraperitoneal administration. Method: The crude polysaccharides were separated and purified by tangential-flow ultrafiltration, then the relative molecular weight was measured by gel column chromatography. The structure was analyzed by pre-column derivatization HPLC. The activity of the homogeneous polysaccharide was investigated by carbon clearance on immunosuppression mice. Result: A new homogeneous polysaccharide named ODP-1 was obtained from O. diffusa. The relative molecular weight of ODP-1 was about 20.88 kDa. ODP-1 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose with the molar ratio of 0.005: 0.033: 0.575: 1.000: 0.144: 0.143.Immune activity indicated that ODP-1 can improve the clearance index, phagocytic index, the index of thymus and spleen of immunosuppression mice. Conclusion: ODP-1 from O. diffusa is a homogeneous polysaccharide with immuno-enhancing activity.