HPLC-DAD测定厚朴中6种活性成分的含量

Determination of Six Active Compounds in Commercial Samples of Magnoliae Officinalis Cortex by HPLC-DAD

摘要:

目的: 以6种活性成分的含量为指标评价厚朴商品的质量.方法: 建立了HPLC-DAD对厚朴样品中4种极性成分木兰花碱、紫丁香苷、木兰苷A及木兰苷B的含量测定方法,流动相醋酸水(pH 3.0)-甲醇梯度洗脱,流速1 mL ·min^-1,检测波长265,328 nm,柱温35 ℃.参照本课题组前期报道的方法测定了脂溶性成分厚朴酚、和厚朴酚的含量.结果: 所建立4种极性成分含量测定方法平均加样回收率范围97.63%~103.84%,RSD 2.41%~4.55%.厚朴中各类成分的含量由高至低顺序为厚朴酚类、木兰苷类、木兰花碱和紫丁香苷.筒皮极性成分中除紫丁香苷外,其他成分含量均高于饮片(P<0.05);部分药材中厚朴酚与和厚朴酚含量未达到2010年版《中国药典》的要求.结论: 所建立的厚朴样品中4种极性成分含量测定方法准确、可靠.不同等级厚朴商品质量不同,总体来说,"选货"中活性成分的含量较"统货"高.

Abstract:

Objective:The study aimed to evaluate the quality of commercial samples of Magnoliae Officinalis Cortex based on the contents of six active compounds. Method: An HPLC-DAD method was established to determine the contents of four polar constituents, magnoflorine, syringin, magnoloside A, magnoloside B. The HPLC separation was carried out on an Agilent Zorbax C₁₈ column (4.6 mm×250 mm, 5 μm) with a mixture solution of water-acetic acid (pH 3.0) and methanol as the mobile phase applied in a gradient elution at a flow rate of 1 mL · min^-1. The wavelength was set at 265 nm for magnoflorine and syringin and 328 nm for the two magnolosides. The contents of magnolol and honokiol were determined using our previous method. Result: The average recovery of the four polar compounds ranged from 97.63% to 103.84% with RSDs less than 5.00%. The contents of honokiol/magnolol, magnolosides, magnoflorine, syringin in samples decreased in turn. The contents of polar compounds except syringin in the bark samples were higher than those in the decoction pieces (P<0.05). Contents of magnolol and honokiol in some samples didn't reach the requirement in Chinese Pharmacopoeia (2010 edition). Conclusion: The developed HPLC method is accurate and reliable. Generally speaking, the thick samples with good appearance possess higher contents of the active constituents than those thin and edge-damaged ones.