HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量

Simultaneous Determination of Plantamajoside,Polydatin,Specnuezhenide and Resveratrol in Huzhentongfeng Capsule with HPLC

摘要:

目的: 建立同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇含量的HPLC方法.方法: 采用高效液相法测定,色谱条件为Agilent SB-C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.1%甲酸溶液梯度洗脱,检测波长280 nm,流速1.0 mL ·min^-1,柱温30 ℃.结果: 大车前苷、虎杖苷、特女贞苷、白藜芦醇分别在0.099 6~1.992 (r=0.999 9),0.123~2.46 (r=0.999 9),0.145~2.90 (r=0.999 9),0.025~0.50 μg(r=0.999 8)与峰面积呈良好的线性关系,平均回收率分别为99.27%,99.43%,98.83%,98.07%.结论: 该方法快速简便,重复性好,专属性强,可作为虎贞痛风胶囊的质量控制方法.

Abstract:

Objective:The aim of this study was to establish a HPLC method for simultaneous determination of the contents of plantamajoside, polydatin, specnuezhenide and resveratrol in Huzhentongfeng capsules. Method: HPLC was used to determine the contents. The analytical column was Agilent SB-C₁₈ column (4.6 mm×250 mm, 5 μm). The liquid phase was composed of acetonitrile and 0.1% formic acid with gradient elution. The detection wavelength was set at 280 nm, the flow rate was 1.0 mL · min^-1 and the column temperature was maintained at 30 ℃. Result: The mass of plantamajoside, polydatin, specnuezhenide and resveratrol with peak areas were within the concentration ranges of 0.099 6-1.992 μg(r=0.999 9), 0.123-2.46 μg(r=0.999 9), 0.145-2.90 μg(r=0.999 9), 0.025-0.50 μg(r=0.999 8), and the average recoveries were 99.27%, 99.43%, 98.83%, 98.07%, respectively. Conclusion: The method is rapid and simple, highly reproducible and specific so that it could be used as one of the quality control methods of Huzhentongfeng capsules.