HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量

Determination of Rosemary Acid and Luteolin in Defatted Perillae fructus by HPLC

摘要:

目的: 建立HPLC同时测定脱脂紫苏子中迷迭香酸和木犀草素含量的方法.方法: 采用Diamonsil C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-0.1%磷酸溶液(55:45),流速1 mL ·min^-1,检测波长330,350 nm,柱温30 ℃.结果: 迷迭香酸在9.91~158.55 mg ·L^-1回归方程为Y=108.83X+0.14 (r=0.999 9),平均回收率为99.68%,RSD 2.01%;木犀草素在0.997~15.952 mg ·L^-1回归方程为Y=119.63X-3.68(r=0.999 9),平均回收率为100.34%,RSD 2.86%.结果: 该方法操作简便、准确,重复性良好,可用于测定脱脂紫苏子中迷迭香酸和木犀草素的含量.

Abstract:

Objective:The aim of this study was to establish an HPLC method for simultaneous determination of the contents of Rosemary acid and Luteolin in defatted Perilla. Method: Diamonsil C₁₈ (4.6 mm×250 mm, 5 μm) column was used, with methanol-0.1% phosphoric acid(55: 45) as mobile phase. The flow rate was set at 1.0 mL · min^-1, the detection wavelength was set at 330 and 350 nm, with the column temperature at 30 ℃. Result: The linear response ranges were 9.91-158.55 mg · L^-1 with regression curve Y=108.83X+0.136 8 (r=0.999 9) for rosemary acid, and 0.997-15.952 mg ·L^-1 with regression curve Y=119.63X-3.677 8(r=0.999 9) for luteolin. The average recovery of rosemary acid and luteolin were 99.68% and 100.34%, respectively (RSD 2.01%, 2.86%). Conclusion: A simple, accurate and reproducible HPLC method has been provided for simultaneous determination of rosemary acid and luteolin in defatted Perilla.