基于荧光光谱法的中药凉茶颗粒的快速判别分析

Rapid Discriminant Analysis of Herbal Tea Particles Based on Fluorescent Spectroscopy

摘要:

目的: 建立快速鉴别中药凉茶颗粒的方法.方法: 测定凉茶颗粒样品的二维及三维荧光光谱,提取图谱特征信息(λ_Ex,λ_Em,I_Ex,I_Em),比较不同品种、不同厂家及不同批次凉茶颗粒荧光光谱图的差异;利用SPSS 17.0软件对不同品种及不同厂家的产品进行分类及鉴别.结果: 不同品种及不同厂家的凉茶颗粒荧光光谱图差异显著,而同品种同厂家不同批次的则差异极小;不同品种及不同厂家凉茶颗粒的判别分析结果与实际结果完全吻合.结论: 结合荧光光谱法与计算机模式识别用于中药凉茶颗粒的分类和鉴别,方法简便、快捷、准确.

Abstract:

Objective:The study aimed to establish a convenient analytic method to identify herbal tea particles from different kinds or sources. Method: The two-dimensional and three-dimensional fluorescence spectra of 35 herbal tea particle samples were determined, and the characteristics and differences of the spectra were comparatively analyzed. At the same time, the characteristic spectra parameters. λ_Ex, λ_Em, I_Ex and I_>Em, were extracted. The analysis was performed using SPSS 17.0. Result: There were significant differences exhibited in their fluorescence spectrum of herbal tea particles from different kinds and sources. However, slight differences of fluorescence spectra were observed in different production batches of herbal tea particle in the same type from the same factory. The identification analysis matched well with the actual results. Conclusion: The fluorescence spectroscopy combining with computer pattern recognition is a useful method in the rapid identification of herbal tea particles.