苦豆子种子生物碱类化学成分及细胞毒活性

Alkaloids Constituents of Sophora alopecuroides Seed and Their Cytotoxic Activity

摘要:

目的: 研究苦豆子种子的化学成分并对其抗肿瘤活性进行测试.方法: 采用常规的硅胶柱层析(CC),葡聚糖(Sephadex LH-20)柱层析,和重结晶等实验手段对其进行了分离纯化,利用波谱技术(¹H-NMR, ¹³C-NMR)及与文献数据对照,对分离的化合物进行了结构鉴定;并采用 MTT 测定部分生物碱单体化合物体外对C6肿瘤细胞的细胞毒活性.结果: 共分离出7个生物碱类化合物,分别鉴定为:苦参碱(1),氧化苦参碱(2),槐果碱(3),9α-羟基槐果碱(4),氧化槐果碱(5),9α-羟基槐胺碱(6),(-)-13,14-去氢槐定碱(7),1个非生物碱化合物3-吲哚甲醛(8).结论: 化合物8为首次从该属植物中分离得到.细胞毒活性测试结果表明,化合物1~3和6对鼠脑胶质瘤C6细胞显示了较强的生长抑制活性.

Abstract:

Objective:To investigate the chemical constituents in seed of Sophora alopecuroides and to determine their antitumor activities in vitro. Method: Compounds were isolated and purified by means of silica gel and Sephadex LH-20 gel columm chromatography, and recrystallization etc. Their structures were elucidated using spectroscopic analysis including ¹H-NMR, ¹³C-NMR and spectral data. The cytotoxic activities of the alkaloids compounds on C6 cell in vitro were evaluated by MTT method. Result: Seven compounds were isolated from the ethanol extract of the seed of S. alopecuroides. Their structrues were identified as (+)-matrine(1), (+)-oxymatrine(2), (-)-sophocarpine(3), (-)-9α-hydroxysophocarpine(4), (+)-oxysophocarpine(5), (-)-9α-hydroxysophoramine(6), (-)-13, 14-dehydrosophoridine(7), and a non-alkaloid named indolyl-3-carbaldehyde(8). Conclusion: The compond 8 was isolated from the genus sophora for the first time. Compounds 1-3 and 5 showed significant inhibitory activity towards C6 glioma cells.