HPLC同时测定牛皮杜鹃中3种成分的含量及其提取方法的比较

Simultaneous Determination of Three Components in Rhododendron chrysanthum by HPLC and Comparison Among Different Extraction Method

摘要:

目的: 建立HPLC同时测定长白山牛皮杜鹃中芦丁、槲皮素及山柰酚含量的方法,并比较不同提取方法对其含量的影响.方法: 采用Agilent Eclipse XDB-C₁₈色谱柱(4.6 mm×250 mm, 5 μm);乙腈-0.1%磷酸水溶液进行梯度洗脱;检测波长为360 nm,流速为0.8 mL ·min^-1,柱温为30 ℃.结果: 芦丁、槲皮素、山柰酚的在一定范围内线性关系均良好;平均加样回收率分别为99.9%(RSD 1.2%),99.2%(RSD 2.8%),101.8%(RSD 2.3%).结论: 该方法简便、快速、准确可靠.3种不同提取方法中以超声提取法所得黄酮类含量均最高.可用于长白山牛皮杜鹃的质量控制.

Abstract:

To establish an HPLC method for the simultaneous determination of rutin, quercetin and populnetin in Rhododendron chrysanthum from Changbai mountain and compare their contents by different extractive methods. The HPLC equipped with the Agilent Eclipse XDB-C₁₈column (4.6 mm×250 mm, 5 μm). The mobile phase consisted of acetonitrile and 0.1% phosphoric acid with gradient elution. The detection wave length was set at 360 nm and the flow rate was 0.8 mL · min^-1, and the temperature was set at 30 ℃. The linear ranges of rutin, quercetin and populnetin were good, the average recoveries were 99.9%(RSD 1.2%), 99.2%(RSD 2.8%), 101.8%(RSD 2.3%), respectively. The method is simple, quick, accurate and repeatable for quantitative analysis. The supersonic extraction showed the highest content of flavonoids among the three extractive methods. The method can be used for quality control of R. chrysanthum from Changbai mountain.