爱罗咳喘宁对慢性阻塞性肺疾病大鼠肺功能、血气指标及病理变化的影响

Study of Effects Lung Function,Blood Gas Indexes and Pathological Changes of Ailuo Kechuanning on COPD Model Rats

摘要:

目的: 制备慢性阻塞性肺疾病(chronic obstructive pulmonarydisease,COPD)大鼠模型,观察肺功能、血气指标、病理变化及爱罗咳喘宁对其影响.方法: 采用脂多糖(lipopolysaccharide,LPS)加烟雾诱导COPD大鼠模型,大鼠随机分为正常组、模型组、爱罗咳喘宁低、中、高剂量组.正常组、模型组ig生理盐水(15.52 mL ·kg^-1 ·d^-1),爱罗咳喘宁低、中、高剂量组(7.75,15.52,31.04 g ·kg^-1 ·d^-1,ig),连续 14 d.评价COPD大鼠模型,观察爱罗咳喘宁对COPD大鼠模型肺功能、血气指标及病理变化的影响.结果: 与正常组比较,模型组用力肺活量(FVC),1秒用力呼气量(FEV₁),FEV₁/FVC,用力呼气流量(FEF)FEF25-75,最大呼气中期流速(MMF),呼气流量峰值(PEF)和动脉血酸碱度(pH)、动脉血氧分压(PaO₂),动脉血氧饱和度(SaO₂)均显著降低,动脉血二氧化碳分压(PaCO₂)显著升高(P<0.05,P<0.01).与模型组比较,爱罗咳喘宁中剂量组FVC,FEV₁,FEV₁/FVC,FEF25-75,MEFV,PEF和pH,PaO₂,SaO₂均显著升高(P<0.05),PaCO₂显著降低(P<0.05).结论: COPD大鼠模型制备成功,爱罗咳喘宁能改善肺组织结构,提高肺功能.

Abstract:

Objective:To study the effect of Ailuo Kechuanning on therapeutic effect of rats with chronic obstructive pulmonarydisease(COPD). Method: Lipopolysaccharide(LPS) smoke was used to COPD rat model; the experimental animals were randomly divided into normal control group, model group, Ailuo Kechuanning group(7.75, 15.52, 31.04 g · kg^-1 · d^-1).The normal control group, model group were given intragastrically the normal saline solution, while other groups were given corresponding herbal drugs intragastrically(7.75, 15.52, 31.04 g · kg^-1 · d^-1) for 14 days.Study of effects lung function, blood gas indexes and pathology change of Ailuo Kechuanning in the rats of all groups before and after treatment. Result: The levels of forced vital capacity(FVC), forced expiratory volume in 1second(FEV₁), FEV₁/FVC, forced expiratory flow (FEF)25-75, maximum midexpiratory flow(MMF), peak expiratory flow(PEF) and potential of hydrogen(pH), arterial oxygen pressure(PaO₂), arterial oxygen saturation(SaO₂) were markedly lower, while partial pressure of carbon dioxide in artery (PaCO₂)keep higher in the model group compared with those in the normal control group(P<0.05, P<0.01).The levels of FVC, FEV₁, FEV₁/FVC, FEF25-75, MEFV, PEF and pH, PaO₂, SaO₂ were markedly higher, but PaCO₂ were lower in the Ailuo Kechuanning group (15.52 g · kg^-1 · d^-1 than those in model group, lung tissue inflammation significantly reduce. Conclusion: Theeffects of Ailuo Kechuanning for treatment of COPD may improve the pulmonary function and protect the lung tissue.