组分配伍四逆汤对甲状腺功能减退症大鼠脑神经递质的影响

Neurotransmitter Influences of Component Comptibility Sini Tang to Hypothyroidism

摘要:

目的: 研究组分配伍四逆汤对甲状腺功能减退症脑神经递质的影响.方法: 将96只大鼠随机分为空白组、模型组、优甲乐阳性对照组(0.01 g ·kg^-1),组分配伍四逆汤高、中、低剂量组(9.6,4.8,2.4 g ·kg^-1).除空白组外,其他组连续灌胃丙基硫氧嘧啶(PTU)溶液0.01 g ·kg^-1 15 d 造甲减模型,用组分配伍四逆汤高、中、低剂量和优甲乐对此动物模型进行治疗,连续给药1个月,治疗同时隔日给予1%PTU维持甲减状态,实验结束后,取脑组织,做10%脑匀浆,用酶联免疫吸附法测去甲肾上腺素(NA),5-羟色胺(5-HT)和多巴胺(DA)浓度.结果: 模型组与空白组相比,其NA浓度明显降低(P<0.05),5-HT浓度显著升高(P<0.01),DA浓度明显降低(P<0.05).与模型组相比,四逆汤高、中剂量、阳性药组,NA浓度有显著升高(P<0.01);四逆汤低、中、高剂量组、阳性对照组,5-HT浓度明显降低(P<0.05,P<0.01);四逆汤高、中剂量、阳性药组,DA浓度显著升高(P<0.01).结论: 四逆汤对甲状腺功能减退症脑内神经递质有一定的调节作用.

Abstract:

Objective:To study the brain neurotransmitter influences of component comptibility of Sini Tang(CCSNT) to hypothyroidism. Method: The hypothyroidism mode was made by 1% propylthiouracil (PTU) solution 15 days, treat the animal which has hypothyroidism with CCSNT of high, middle, low dose (9.6, 4.8, 2.4 g · kg^-1, ig) and positive drugs Euthyrox 0.01 g · kg^-1, ig, at the same time the mice should give the PTU(0.01 g · kg^-1, ig);after the end of the experiment, take the brain and make 10% brain homogenates, use the enzyme-linked immunosorband assay (ELISA) kit to measure the norepinephrine(NA), 5-hydroxytryptaphane(5-HT)and dopamine(DA) concentration. Result: Compared with the blank group, in the model group the concentration of NA and DA are decreased(P<0.05), concentrations of 5-HT is significantly increased (P<0.01). Compared with model group, the concentration of NA and DA of CCSNT high dose, middle dose and the Euthynx group, are significantly increased (P<0.05);the 5-HT level of all the treatment group are decreased(P<0.05). Conclusion: CCSNT has same effect of Sini Tang to neurotransmitters of hypothyroidism.