补阳还五汤对博莱霉素所致肺纤维化大鼠肺指数及肺组织病理的影响

Effects of Buyang Huanwu Tang on Lung Index and Pathomorphology of Pulmonary Fibrosis Rats by Bleomycin

摘要:

目的: 观察补阳还五汤对博莱霉素致肺纤维化大鼠肺指数的影响.方法: 将144只SD大鼠随机分成6组,即假手术组、模型组、强的松(4.2 mg ·kg^-1)组、补阳还五汤高、中、低剂量组(18.48,9.24,4.62 g ·kg^-1),每组各24只.除假手术组外,其余各组采用一次性气管滴注博莱霉素复制肺纤维化大鼠模型,假手术组气管内滴注等量生理盐水,于造模第2天起,各给药组分别给予相应药物灌胃,动态观察模型大鼠在实验期间的一般情况(包括精神、摄食、毛色、活动等),于造模后第7,14,28天,分3批处死动物,分离肺组织并计算肺指数;取左肺制备病理切片,行HE染色观察肺泡炎及肺纤维化程度改变情况.结果: ①肺指数:与假手术组相比,模型组大鼠各时间点肺指数增加明显(P<0.01);与模型组比较,各给药组大鼠肺指数有不同程度的下降(P<0.01);与强的松对照组相比,补阳还五汤中剂量组第14,28天及低剂量组第28天肺指数降低较明显(P<0.05);高剂量组3个时间点肺指数均高于强的松对照组,但与同期模型组相比仍较有明显的降低.②肺组织形态学:与假手术组相比,模型组及各给药组大鼠可见肺泡结构有不同程度的破坏,肺间质增生,有炎细胞浸润和胶原纤维增生形成;与模型组相比,给药组各时间点的肺泡炎和肺纤维化程度均有不同程度的改善(P<0.01);其中补阳还五汤中剂量组在中后期降低肺泡炎及纤维化程度方面明显优于同期强的松对照组(P<0.05).结论: 补阳还五汤能够较好地控制模型大鼠肺指数的增长,抑制早期的肺泡炎和中后期的肺纤维化,其具体机制有待进一步研究.

Abstract:

Objective:To investigate the effects of Buyang Huanwu Tang on lung index of pulmonary fibrosis(PF) rats induced by bleomycin (BLM). Method: Nintys healthy male SD rats were randomly divided into six groups:sham operation group, model group, prednisone group(4.2 mg · kg^-1), Buyang Huanwu Tang high-dose, middle-dose, low-dose groups(18.48, 9.24, 4.62 g · kg^-1), 24 rats in each group. Pulmonary fibrosis model was established by injected with BLM by tracheal intubation, sham operation group was intratracheally instilled with equal NS, then every rats was raised drugs through a gastric tube one times a day. To observing general status of PF rats (such as the condition of mental, diet, hair, behavioral activity), the changes of lung index, to measure alveolar catarrh and pulmonary fibrosis degrees in left lung dyed with HE. Result: ①Lung index:at all time points, a significant increase in lung index of model group were higher than that of sham operation group (P<0.01);other medicated groups had different decreases in lung index by comparison with model group (P<0.01);compared with the prednisone group, the lung index of Buyang Huanwu Tang middle-dose group showed an obviously decreased tendency on the 14^th and 28^th day (P<0.05), this condition of that in Buyang Huanwu Tang low-dose group on the 28^th day (P<0.05), Buyang Huanwu Tang high-dose group than in prednisone group the lung index of some high at there time points, but were significantly lower than the same period in the model group. ②Pathomorphology:compared with the sham operation group, the model group and each medicated groups were mainly featured with destroyed alveoli, thickened alveoli septum and the deposition of collagen fibers on different level;the medicated groups could improve the alveolar catarrh and pulmonary fibrosis on pathomorphology in varying degree by comparison with the model group (P<0.01);the effect of reducing alveolar catarrh and pulmonary fibrosis of Buyang Huanwu Tang middle-dose group had an advantage over that of the prednisone group on the 14^th and 28^th day (P<0.05). Conclusion: Buyang Huanwu Tang could obviously control the growth of lung index, and alleviate alveolar catarrh and pulmonary fibrosis, the specific mechanism of that were expecting for further study.