消癥丸对小鼠黄褐斑模型的作用及机制

Effect and Mechanism of Xiaozheng Wan for Mouse Melasma Model

摘要:

目的: 观察消癥丸对肌肉注射黄体酮复合紫外照射致小鼠黄褐斑模型的抗氧化与抗黄褐斑形成作用.方法: 雌性ICR小鼠随机分为正常组、模型组、百消丹组(3 g ·kg^-1,ig)、消癥丸低、中、高剂量组(1,2,4 g ·kg^-1,ig),采用黄体酮 (20 mg ·kg^-1,im),同时配合320 nm 的中波紫外线照射,每天1 h,连续36 d,造成小鼠黄褐斑模型.实验结束后,测定各组肝脏和皮肤中的酪氨酸酶(TYR)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量,另取照射部位皮肤进行病理学检查及免疫组化方法测定皮肤黑色细胞数.结果: 与正常组比较,模型组肝脏和皮肤中TYR含量明显升高,具有显著性差异(P<0.01);给予百消丹、消癥丸后肝脏和皮肤中的TYR含量均明显降低,与模型组相比,具有显著性差异(P<0.01);同时,皮肤中MDA含量降低,与模型组相比,具有显著性差异(P<0.01);而皮肤和肝脏中的SOD以及肝脏中的MDA则没有显著差异.结论: 消癥丸有一定的防治黄褐斑形成的功效,其作用与抑制TYR活性,减少黑色素细胞的生成有关.

Abstract:

Objective:To observe the effect and mechanism of Xiaozheng wan for mouse melasma model of progesterone composed of UV radiation induced. Method: Female ICR mice were randomly divided into normal group, model group, Baixiao dan group (3 g · kg^-1), Xiaozheng Wan low-dose group (1 g · kg^-1), middle dose group (2 g · kg^-1), high-dose group (4 g · kg^-1), except the normal group and the model group was given normal saline, the rest of the group at the suggest doses administered by gavage for 36 days. And at the beginning of the administration except the normal group, the other groups were using progesterone intramuscular injection (20 mg · kg^-1), while 320 nm ultraviolet irradiation of one hour, once a day for 36 days, to cause chloasma mouse model. After the end of the experiment, measuring liver and skin tyrosinase (TYR), superoxide dismutase (SOD), malondialdehyde (MDA) content, the other to take part irradiated skin pathological and immunohistochemical determination of skin black cells. Result: The treatment group significantly reduced liver and skin TYR content, compared with model group with a significant difference (P<0.01);skin can reduce the content of MDA, but no significant difference;effect on the skin and liver SOD is low. Conclusion: Xiaozheng wan chloasma composite model in mice can inhibit melanin production role.