热毒宁注射液抗大鼠急性肺损伤治疗作用

Effect of Reduning Injection on Acute Lung Injury of Rats

摘要:

目的: 考察热毒宁注射液(RDN)对脂多糖(LPS)致大鼠急性肺损伤(ALI)的治疗作用及其机制研究.方法: SD大鼠60只,将大鼠随机分成6组,每组10只,即正常组、模型组、阳性药组(地塞米松2 mg ·kg^-1)、热毒宁注射液高、中、低剂量(10.16,5.08,2.54 g ·kg^-1)组.除正常组外采用LPS按6 mg ·kg^-1对SD大鼠气管内滴注制作急性肺损伤模型,观察受试药热毒宁注射液3个剂量组和阳性药地塞米松注射液(DEX)iv给药后对肺脏湿/干重(W/D)、支气管肺泡灌洗液(BALF)总蛋白含量和血清超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)活性,丙二醛(MDA)含量及肺组织匀浆白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)含量的影响.结果: 与正常组比较,模型组大鼠W/D升高、BALF总蛋白含量升高、MDA含量和MPO活性升高,SOD活性降低,IL-1β,TNF-α,IL-10水平升高(P<0.01),与模型组比较,热毒宁注射液不同剂量均降低大鼠W/D,降低BALF总蛋白含量、降低MDA含量和MPO活性,增强SOD活性,降低IL-1β,TNF-α,IL-10水平(P<0.01,P<0.05).结论: RDN可改善肺组织病理变化,减轻LPS致ALI 过程中体内的氧化应激反应,减少炎性因子可能是治疗急性肺损伤疾病的重要机制.

Abstract:

Objective:To investigate the mechanism and the rapeutic effect of Reduning injection(RDN) on acute lung injury(ALI) in rats. Method: Sixty rats were randomly divided into six groups, normal group, model group, positive control group (dexamethasone, 2 mg · kg^-1), high-, medium-, low-dose RDN groups (10.16, 5.08, 2.54 g · kg^-1).The ALI rats was induced by ipopolysaccharide(LPS) except for rats in normal group. The effects of wet /dry weighto (W/D) ratio in lung, the total protein contents of bronchoalveolar lavage fluid (BALF), superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO) in serum and interleukin(IL) -1β, tumor necrosis factor (TNF) -α, IL-10 in lung homogenate were observed. Result: The W/D ratio, BALF, the content of MDA, IL-1β, TNF-α, IL-10 were decreased, the activity of MPO was inhibited and the activity of SOD was activated in model group. The above indexes were all improved in all dosage of RDN groups(P<0.01, P<0.05). Conclusion: RDN can improve the pathological changes of lung tissueand reduce the oxidative stress reaction of ALI process. Its mechanism may be achieved by reducing the inflammatory factor in vivo.