甘木通总黄酮抗心肌缺血的作用

Protective Effect of Total Flavones from Clematis filamentosa on Myocardial Ischemia

摘要:

目的: 研究甘木通总黄酮抗心肌缺血作用.方法: 取Wistar大鼠36只,随机分为6组,每组6只,假手术组、模型组、甘木通总黄酮低、中、高剂量组(8,16,32 mg ·kg^-1)和复方丹参片组(250 mg ·kg^-1),除假手术组外,其余大鼠采用结扎大鼠冠状动脉前降支制备心肌缺血模型,造模成功后ig给药,观察甘木通总黄酮对大鼠心电图ST段位移、血清心肌酶活力及心肌梗死范围的影响;昆明种小鼠30只,随机分为5组,分别为生理盐水组、甘木通总黄酮低、中、高剂量组(12,24,48 mg ·kg^-1)和复方丹参片组(360 mg ·kg^-1),采用小鼠密闭缺氧实验,观察甘木通总黄酮对小鼠耐缺氧存活时间的影响.结果: 与假手术组比较,模型对照组心电图ST段即刻出现显著性抬高(P<0.01),血清谷草转氨酶(AST),乳酸脱氢酶(LDH),肌酸肌酶同工酶(CK-MB)活力均显著升高(P<0.01),心肌梗死范围显著升高(P<0.01);与模型组比较,甘木通总黄酮各剂量组均可不同程度地降低急性心肌缺血大鼠不同时间点心电图ST段抬高值(P<0.05),降低血清AST,LDH,CK含量(P<0.05,P<0.01),降低心肌梗死率(P<0.01),并呈现一定的剂量效应;还可显著延长小鼠常压耐缺氧时间(P<0.05).与复方丹参片组比较均无差异.结论: 甘木通总黄酮对心肌缺血具有显著的保护作用.

Abstract:

Objective:To determine the protective effect of total flavones from Clematis filamentosa (TFCF) on myocardial ischemia (MI). Method: Thirty-six rats were randomly divided into the sham operation group, model control group, compound danshen tablet group (250 mg · kg^-1), low-, moderate- and high-dose TFCF groups (8, 16, 32 mg · kg^-1). MI was induced in rats by ligation of left anterior descending coronary artery except for rats in the sham operation group.Changes of segment ST of electrocardiogram, myocardial enzyme activity, and myocardial infarction area were observed. Thirty mice were randomly divided into the normal saline group, compound danshen tablet group (360 mg · kg^-1), low-, moderate- and high-dose TFCF groups (12, 24, 48 mg · kg^-1). Hypoxia tolerance test was performed by placing the mice into the closed bottle and their survival time was measured. Result: Compared with the model control group, TFCF could reduce ST segment elevation at different time points (P<0.05), decrease the serum levels of aspartate transaminase(AST), lactate dehydrogenase(LDH), and creatine kinase-MB(CK-MB) (P<0.05, P<0.01), and reduce the rate of MI in acute myocardial ischemia rats (P<0.01). A certain dose-effect relationship was observed. TFCF also could remarkably prolong the mouse's survival time in hypoxia test (P<0.05). No significant difference was found between TFCF and compound danshen tablet. Conclusion: TFCF is proved to have an obvious protective effect on the experimental MI model.