响应面法优化番石榴叶总三萜的超声提取工艺

Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of Psidium guajava by Response Surface Methodology

摘要:

目的: 优选番石榴叶中总三萜超声提取工艺. 方法: 以总三萜提取率为指标,在单因素试验基础上,通过响应面法考察乙醇体积分数、料液比、超声功率对总三萜提取率的影响.采用UV测定总三萜含量,检测波长540 nm. 结果: 最佳提取工艺条件为加60倍量70%乙醇于150 W,50℃ 超声提取2次,每次30 min.总三萜提取率7.93%,与理论值7.86%偏差较小. 结论: 通过响应面分析法优选番石榴叶总三萜提取工艺可行性较好,为该植物部位的开发与利用提供实验依据.

Abstract:

Objective: To optimize extraction process of total triterpenoids from leaves of Psidium guajava. Method: Considering yield of total triterpenoids as index,based on single factor experiments,response surface methodology was adopted to optimize extraction process by taking ethanol concentration,liquid-solid ratio and ultrasonic power as factors.UV was employed to determine the content of total triterpenoids at 540 nm. Result: Optimum technological conditions were as follows:extracted twice with 60 times the amount of 70% ethanol at 150 W and 50℃ for 30 min of per time;yield of total triterpenoids was 7.93%,whose deviation was small by comparing with the theoretical value of 7.86%. Conclusion: Response surface analysis used in optimization of total triterpenoids is feasible,this study can provide experimental basis for development and utilization of this plant part.