不同炮制工艺山绿茶药材特征图谱的比较

Comparative on Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis

摘要:

目的: 采用HPLC研究不同炮制工艺山绿茶药材特征图谱的变化,为科学评价和控制其质量提供有效的方法. 方法: 以70%乙醇为提取溶剂,采用加热回流的方法提取制备供试样品.以Phenomenon C₁₈色谱柱为固定相,流动相乙腈-0.1%磷酸水梯度洗脱,检测波长330 nm,柱温25℃,进样量10 μL,流速1.0 mL·min^-1,进行HPLC特征图谱检测.以芦丁为对照品,检测不同炮制工艺山绿茶药材中芦丁含量变化. 结果: 不同炮制工艺药材中共确定9个主要共有特征峰.研究发现特征图谱中主要色谱峰发生了显著改变,与炮制前相比峰面积显著降低.表明山绿茶药材经炮制处理后,主要化学成分含量明显减少. 结论: 该HPLC特征图谱检测方法重复性、精密度良好,可用于山绿茶药材及其炮制品的质量控制.

Abstract:

Objective: The aim of this study was to establish a sensitive and specific HPLC method for quality control of Ilex hainanensis. Method: HPLC method was applied for quality assessment of I. hainanensis. HPLC analysis was performed on a phenomenon C₁₈ column (4.6 mm×250 mm,5 μm).The mobile phase was consisted of acetonitrile (solvent B) and watercontaining 0.1% phosphoric acid (solvent A) at a constant flow rate of 1.0 mL· min^-1.An increasing linear gradient of solvent B was used. The injection volume was 10 μL. The column temperature was set at 25℃. The chromatograms were monitored at 330 nm. Result: In I. hainanensis obtained from different processing methods, there were 9 characteristic peaks identified. It was established from its prepared samples of I. hainanensis. Conclusion: The characteristic chromatogram of I. hainanensis with high specificity can be used to control its quality as well as the uniformity of processing products.