广西产毛果算盘子的HPLC指纹图谱

HPLC Fingerprints of Glochidion eriocarpum in Guangxi

摘要:

目的: 采用HPLC研究广西不同产地毛果算盘子的指纹图谱. 方法: 采用Hypersil C₁₈柱(4.6 mm×250 mm,5 μm),流动相甲醇(A)-0.2%磷酸(B)梯度洗脱(0~7 min,10%A;7~15 min, 10%~22%A;15~20 min,22%~30%A;20~30 min,30%~40%A;30~45 min,40%A),检测波长270 nm,柱温20℃,流速1.0 mL·min^-1,分析时间45 min.分析了10批毛果算盘子药材的HPLC指纹图谱.应用中药色谱指纹图谱相似度评价系统(2004A版)计算相似度. 结果: 建立了广西产毛果算盘子的指纹图谱,且相似度较好,根据相似度分析确定了9个构成毛果算盘子药材指纹图谱的特征峰. 结论: 该方法简单、稳定、重复性好,可用于毛果算盘子药材的质量控制.

Abstract:

Objective: The study aimed to establish the chromatographic fingerprint of Glochidion eriocarpum by HPLC. Method: The fingerprint was investigated using Hypersil C₁₈ (4.6 mm×250 mm,5μm) column. The mobile phase was consisted of methanol (A) and 0.2% phosphoric acid (B), in gradient elution for 0-7 min,10%A;7-15 min, 10%-22%A;15-20 min,22%-30%A;20-30 min,30%-40%A;30-45 min,40%A. The detection wavelength was set at 270 nm, and column temperature was set at 30℃, The flow rate was 1 mL· min^-1. The fingerprints of 10 batches of G. eriocarpum were analyzed in 45 min. The similarity was analyzed by similarity evaluation system for chromatographic fingerprint of TCM (version 2004 A). Result: The HPLC fingerprint of Guangxi G. eriocarpum was obtained with good similarity. Conclusion: The method was simple, reproducible and can be used in quality control for G. eriocarpum.