固相微萃取与超临界流体萃取艾叶挥发油的GC-MS对比分析

Comparative Analysis GC-MS of Artemisiae Argyi Folium Volatile Oil Extracted by SPME and SFE

摘要:

目的: 探讨固相微萃取(SPME)与超临界流体萃取(SFE)所得南阳艾叶挥发油成分的不同,确定其主含成分及含量,并对其进行分析. 方法: 分别以SPME与SFE提取艾叶挥发油并以GC-MS进行对比分析. 结果: 以SPME法得到了38种化合物,SFE 得到了26种化合物,GC-MS分析分别占其总量的95.17%和95.26%;通过对比质谱数据库(NIST,NBS)进行鉴别,前者鉴别出35个组分,后者鉴别出25个;各组分含量均不相同,但最高者均为1,8-桉树脑(分别为13.75%,18.26%),其他在SPME中依次为2-莰醇(11.58%)、异蒿属(甲)酮(7.15%)及对称二甲基脲(5.12%);在SFE中依次为异蒿属(甲)酮(12.15%),2-莰醇(11.38%)及反-罗勒烯(7.03%). 结论: SPME萃取与SFE萃取所得的艾叶挥发油成分差异较大,相较而言SPME具有较明显的优越性.

Abstract:

Objective: The aim of this study was to analyze the ingredients, and to identify the main ingredients and content of the volatile oil,which extracted from Nanyang Artemisiae Argyi Folium volatile oil by two different solid-phase microextraction (SPME) and supercritical fluid extraction (SFE). Method: The volatile oil was extracted separately from Artemisiae Argyi Folium by SPME and SFE. Then GG-MS was used to analyze it. Result: Thirty-eight components were isolated by SPEM, accounting for 95.17% of the total content,Thirty-five components were with 35 identified by comparing with the mass spectrometry database, while 26 components were isolated by SFE, accounting for 95.26% of the total content, with 25 identified by comparing with the mass spectrometry database. The content of each component varied,with eucaylptole as the most (13.75% and 18.26% respectively). The following components isolated from SPME were including borneol (11.58%), isoartemisia ketone (7.15%) and esym-dimethylurea (5.12%);the following components from SFE included isoartemisia ketone (12.15%), borneol (11.38%) and trans-ocimene (7.03%). Conclusion: The result verified the superiority of the SPME method, though the components of the volatile oil extracted by two different ways are obviously different.