藏族药翼首草的HPLC指纹图谱分析

HPLC Fingerprint of Tibetan Medicine Pterocephali Herba

摘要:

目的: 建立藏药翼首草的高效液相色谱指纹图谱,并对不同产地翼首草的指纹特征进行比较,为其质量评价提供新的方法. 方法: 采用HPLC 测定了10 批不同产地的翼首草样品.Boston Symmetrix色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-0.1 mol·L^-1乙酸铵水溶液(81:19),流速0.8 mL·min^-1,检测波长210 nm,柱温30℃.并对不同产地样品的相似度进行了比较. 结果: 利用《药典会指纹图谱相似度评价系统(2004A版)》建立了翼首草药材的指纹图谱共有模式,标出了11个特征峰.10批样品的相似度均较高. 结论: 该方法具有较好的重复性、精密度、稳定性,可为翼首草的质量控制提供科学依据.

Abstract:

Objective: The aim of this study was to establish the HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare the fingerprints of Pterocephali Herba from different places, in order to provide a new method for its quality evaluation. Method: HPLC method was adopted to determine 10 groups of Pterocephali Herba from different places, with Boston Symmetrix(4.6 mm×250 mm,5 μm)chromatographic column. The mobile phase was methanol-0.1 mol· L^-1 ammonium acetate solution(81: 19) at a flow rate of 0.8 mL· min^-1. The detection wavelength was set at 210 nm, column temperature was maintained at 30℃. And the similarities of Pterocephali Herba from different places were compared. Result: The common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba was established according to the fingerprint similarity evaluation system of Pharmacopoeia Convention (2004A version), and 11 characteristic peaks were marked. The 10 groups of samples had high similarity. Conclusion: This method has good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba.