冷水花抗菌活性部位薄层指纹图谱

TLC Fingerprint Analysis of Pilea aquarum

摘要:

目的: 建立冷水花药材抗菌活性部位薄层色谱指纹图谱.方法: 通过抗菌活性研究确定冷水花药材抗菌活性部位,进而采用薄层色谱法对贵州产10批冷水花药材抗菌活性部位进行薄层色谱比较分析.结果: 冷水花药材乙酸乙酯部位具有抗菌活性.另外,贵州产l0批冷水花药材的乙酸乙酯部位薄层色谱图十分相似,均有8个斑点和对应的8个峰,但峰强度上有差异.结论: 该方法操作简单,稳定,可用于冷水花药材的鉴别及质量控制.

Abstract:

Objective: To establish TLC fingerprint of active antibacterial fraction of Pilea aquarum. Method: The activity fraction of P. aquarum was confirmed by antibacterial activity assay, and then the TLC fingerprint of 10 batches of P. aquarum from Guizhou was analyzed comparatively. Result: The EtOAc extraction of P. aquarum is antibacterial activity. The TLC chromatograms of 10 bat ches were very similar, which had 8 spots and 8 corresponding peaks, but the intensity of each peak was different. Conclusion: The method is simple and stabilized, and can be used for the quality control of P. aquarum Dunn.