HPLC-UV对栝楼各部位中葫芦素B的含量测定

Determination of Cucurbitacin B in Some Parts of Trichosanthes kirilowii by HPLC-UV Method

摘要:

目的: 对栝楼的多个部位进行葫芦素B的含量测定. 方法: 样品以50%甲醇超声提取,采用HPLC-UV测定,ODS色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-水(70:30),流速1.0 mL·min^-1,检测波长230 nm,柱温30℃. 结果: 栝楼的雄根、雌根、茎、叶、幼果、未成熟果实、成熟果实、未成熟果瓤、成熟果瓤、种子、雄花、果柄、未成熟果皮、成熟果皮等部位分别进行了葫芦素B的测定.栝楼各部位中葫芦素B的含量从0到0.231 3 mg·g^-1,均未检测到葫芦素E. 结论: 栝楼各部位中葫芦素B的含量高低不等,除一株三年生雌株栝楼粗大的根中含量高达0.231 3 mg·g^-1外,大部分都低于0.09 mg·g^-1,含量的高低与栝楼的部位、生长年限等因素有关.

Abstract:

Objective: To determine the contents of cucurbitacins B in some parts of Trichosanthes kirilowii Maxim. Method The sample solutions were prepared in 50% MeOH by the supersonic extraction. An HPLC-UV method was performed on ODS column (4.6 mm×250 mm, 5 μm) with a mobile phase of methanol-water(70: 30) at a flow rate of 1.0 mL·min^-1, the detection wavelength was at 230 nm, and column temperature at 30℃. Result Cucurbitacins B and E were determined in male root, female root, stem, leaf, young fruit, unripe fruit, ripe fruit, unripe flesh, ripe flesh, seed, male flower, carpopodium, unripe pericarp, and ripe pericarp. The contents of cucurbitacin B were 0-23.13 mg per 100 g sample of each part of T. kirilowii. Cucurbitacin E was not detected in any parts of T. kirilowii. Conclusion: There are different contents of cucurbitacin B in different parts of T. kirilowii. The content of cucurbitacin B was 23.13 mg per 100 g of a stouk root of female T. kirilowii growth for 3 years and lower than 9 mg per 100 g of other samples. The contents of cucurbitacin B were related to some factors such as the growth period and the part of T. kirilowii.