广西多叶越南槐HPLC指纹图谱的建立及与山豆根HPLC指纹图谱一致性比较研究

Establishment of the HPLC Fingerprint of Sophora tonkinensis var. polyphylla from Guangxi and Comparison of Concordance with HPLC Fingerprint of S. tonkinensis

摘要:

目的: 建立多叶越南槐的HLPC指纹图谱分析方法,为科学评价和有效控制多叶越南槐药材的质量提供参考依据;并通过指纹图谱比较多叶越南槐与山豆根的相似性,为多叶越南槐与山豆根同源性研究提供参考数据. 方法: 采用反相高效液相色谱法,Agilent Eclipse-C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.05 mol·L^-1磷酸二氢钾溶液(用三乙胺调pH 6.4)梯度洗脱,流速1.0 mL·min^-1,检测波长215 nm,柱温30℃,采用《中药色谱指纹图谱相似度评价系统》2004A版软件进行数据处理. 结果: 建立的多叶越南槐指纹图谱有14个共有峰,指认了7个共有峰;各批药材指纹图谱比较相似度大部分在0.90以上,基本符合指纹图谱研究技术的要求;多叶越南槐与山豆根指纹图谱相比较,相似度均在0.90以上. 结论: 该方法精密度、稳定性及重复性较好,特征性及专属性强,可以有效地用于多叶越南槐药材的质量控制,为开发和利用多叶越南槐药材资源提供科学依据,通过多叶越南槐与山豆根指纹图谱的比较,可推测出多叶越南槐与山豆根成分相似,亲缘关系相近.

Abstract:

Objective: to establish the HLPC fingerprint methods of Sophora tonkinensis var.polyphylla and provide reference for scientific evaluation and quality control of S. tonkinensis var. polyphylla herb. To provide reference data for the Homology study of ophora tonkinensis polyphylla and S.tonkinensis through comparing the fingerprints. Method: An HPLC methods was used. An Agilent Eclipse XDB-C₁₈ (4.6 mm×250 mm, 5 μm) column was adopted, and the mobile phase consisted of acetonitrile and 0.05 mol· L^-1 potassium dihydrogen phosphate solution(adjusted with triethylamine pH 6.4) with a gradient elution. The flow rate was 1.0 mL· min^-1, column temperature was 30℃, and detection wavelength 215 nm. A ‘chromatrographic fingerprint evaluation system 2004A edition’ software was used for data processing. Result: The establishment fingerprint of S. tonkinensis var. polyphylla has 14 common peaks and 7 peaks of them were identified using the reference retention time and UV-DAD detector extracted spectra dual qualitative indicators. Most of the compare similarities of fingerprints of each batch herb were above 0.90, in line with the requirements of fingerprint technology research. Comparing with the fingerprint of S. tonkinensis var.polyphylla and S.tonkinensis, the similarity were above 0.90. Conclusion: The precision, stability and reproducibility of the established method was good. The method could be effectively used for quality control of S. tonkinensis var.polyphylla herb and provide a scientific basis for the development and use of the herb. It could be infer that S. tonkinensis var. polyphylla and S.tonkinensis have similar ingredients and close relationships from the compared study of their fingerprints.