侧柏酮对人γδT细胞功能的影响

Effect of Thujone on Human Peripheral Blood γδ T Cells Function in Vitro

摘要:

目的: 探讨侧柏酮对人γδ T细胞功能的影响. 方法: 异戊烯焦磷酸/isopentenyl pyrosphate(IPP)法体外扩增人外周血γδ T细胞.各浓度侧柏酮作用人γδ T细胞48 h,CCK-8法检测其增殖力,流式细胞术测其表型、细胞周期、细胞凋亡及表达CD107a,穿孔素(perforin),粒酶B(granzyme B)的情况. 结果: 300～600 μmol·L^-1侧柏酮明显抑制人γδ T细胞生长(P<0.05);0.036 25～150 μmol·L^-1侧柏酮明显促进其生长(P<0.05),9.4 μmol·L^-1时达增殖最高峰(37.03±0.52)%,且穿孔素表达率明显升高(39.94±1.04)%(P<0.05),余与空白组相比无显著差异. 结论: 9.4 μmol·L^-1侧柏酮明显促进人γδ T细胞增殖并上调其穿孔素的表达.

Abstract:

Objective: To investigate the effect of thujone on human peripheral blood γδ T cells function in vitro. Method: The human peripheral blood γδ T cells were expanded using in vitro isopentenyl pyrosphate method. After culturing with different concentrations of thujone for 48 hours, the growth curve of γδ T cells was tested using CCK-8 method;the cell phenotype, the cell cycle, the cell apoptosis, and the expression of CD107a, perforin, granzyme B were tested using flow cytometer. Result: Thujone could obviously suppress the γδ T cells growth with the concentration range of 300-600 μmol· L^-1(P<0.05), while thujone could proliferate the γδ T cells's growth obviously with the range of 0.036 25-150 μmol· L^-1(P<0.05). The peak of proliferation for thujone was (37.03±0.52)%, and the expression rate of proforin was increased by (39.94±1.04)% at the concentration of 9.4 μmol· L^-1(P<0.05). Conclusion: Thujone can obviously proliferate the γδ T cells and up-regulate the expression of preforin of γδ T cells at the concentration of 9.4 μmol· L^-1.