藿香与广藿香抗氧化活性研究

Antioxidant Activity of Agastache rugosa and Pogostemon cablin

摘要:

目的: 研究藿香和广藿香的茎、叶提取物抗氧化活性,总多酚与总黄酮含量与抗氧化活性的关系,并分析二者药理药效是否具有可替代性. 方法: 采用DPPH法、ABTS法和FRAP方法进行研究. 结果: 发现总黄酮含量与抗氧化总能力有一定相关性(r=0.609 9).通过比较藿香与广藿香的抗氧化能力发现,广藿香乙酸乙酯部位清除DPPH自由基能力最强[IC₅₀(79.6±3.14) mg·L^-1],藿香乙酸乙酯部位清除ABTS自由基能力最强[IC₅₀ (4.41±0.23) mg·L^-1],广藿香石油醚部位Fe³⁺的还原能力最强[TEAC (1 822.99±1 141.13) μmol·g^-1]. 结论: 广藿香与藿香均具有一定抗氧化能力,且差别不大.

Abstract:

Objective: To evaluate the antioxidant activity of extracts from Pogostemon cablin and Agastache rugosa. Method: Their antioxidant activity was evaluated and compared using 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) free radical scavenging, 2,2'-amino-di(2-ethyl-benzothiazoline sulphonic acid-6) ammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assay. Result: There was some correlation for antioxidant capacity between the total polyphenol and total flavonoid contents (r=0.6099). The ethyl acetate of P. cablin showed the strongest capacity of DPPH scavenging[IC₅₀(79.60±3.14) mg· L^-1], the ethyl acetate of A. rugosa showed the strongest capacity of ABTS scavenging[IC₅₀(4.41±0.23) mg· L^-1], and the petroleum ether of P. cablin had the strongest capacity of FRAP[TEAC(1 822.99±1 141.13) μmol· g^-1]. Conclusion: They all had certain antioxidant effects and small differences were found between P. cablin and A. rugosa.