左归丸对大鼠血清代谢物质组影响的研究

Studies on Metabolic Substances of Zuogui Wan in Serum of Rats

摘要:

目的: 分析左归丸含药血清物质组的代谢特征. 方法: 采用核磁共振技术(NMR) 获得左归丸大鼠含药血清样本中代谢物指纹图谱的数据,应用正交偏最小二乘判别分析法研究左归丸含药血清与空白血清之间的代谢物谱差异,通过变量重要性投影(VIP)和t检验在血清中发现潜在生物代谢物. 结果: 给左归丸药物后大鼠血清中的丙酮酸,乳酸,低密度脂蛋白和乙酸乙酯等物质对比空白血清有显著差异. 结论: 给左归丸药物后大鼠血清中的丙酮酸,乳酸,低密度脂蛋白和乙酸乙酯等物质的变化,可使能量代谢途径发生变化,并引起机体代谢机能提高.

Abstract:

Objective: To analyze the metabolic characteristics of Zuogui Wan in serum of rats. Method: The technology of nuclear magnetic resonance (NMR) was used to get metabolite fingerprint data of Zuogui Wan in serus, and the orthogonal partial least-squares discriminant analysis method was used to study the difference of the metabolite profile between Zuogui Wan containing serum and blank serum,then the variable importance in projection (VIP) and t test was to show the potential biological metabolites in serum. Result: There were significant differences in the pyruvate, lactic acid, low density lipoprotein and ethyl acetate and other material between Zuogui wan containing serum and blank serum. Conclusion: The variation of pyruvate, lactic acid, low density lipoprotein and ethyl acetate and other material in rats after giving Zuogui Wan containing serum can change the energy metabolism and cause the improvement of metabolism function.