补阳还五汤对阿尔茨海默病小鼠海马形态学和β淀粉样蛋白水平的影响

Effects of Buyang Huanwu Tang on Morphology and β-amyloid of Hippocampal in Alzheimer's Disease Mice

摘要:

目的: 研究补阳还五汤对阿尔茨海默病小鼠海马形态学、脑和脾脏脏器指数、β淀粉样蛋白(Aβ)水平的影响. 方法: 雄性APP/PS1双转基因小鼠随机分为模型组、阳性药吡拉西坦0.62 g·kg^-1组、补阳还五汤高、中、低剂量组(37.06,18.53,9.26 g·kg^-1).以同遗传背景的C57小鼠为正常对照组.ig给予补阳还五汤,连续治疗28 d后取材,观察小鼠海马镜下形态结构、脑和脾脏脏器指数,采用双抗体夹心法测定海马组织Aβ水平. 结果: 与C57对照组比较,模型组小鼠神经元数量减少、发生变性,脑和脾脏的脏器指数明显降低(P<0.05),Aβ水平明显升高(P<0.05);与模型组比较,补阳还五汤组海马神经元细胞形态改善明显,脑和脾脏脏器指数明显升高(P<0.05),Aβ水平明显降低(P<0.05). 结论: 补阳还五汤能维持阿尔茨海默病小鼠神经元的正常形态结构,升高脑和脾脏脏器指数,降低Aβ水平.

Abstract:

Objective: To study effects of Buyang Huanwu Tang (BYHWT)on morphology and organ coefficients of splenic organ and brainand β-amyloid(Aβ) of hippocampal in Alzheimer's disease mice. Method: Male APP/PSI double transgenic mice were randomly divided into control group,model group,treatment group,BYHWT high-dose group(37.06 g· kg^-1), medium dose group(18.53 g· kg^-1), low-dose group(9.26 g· kg^-1).The mice were killed 28 days after continuous treating. After the treatment all animals were sacrificed,morphology of Hippocampal was observed,calculated the organ coefficients of splenic organ and brain. Biochemical methods were used to determine the content of Aβ in the hippocampal tissue. Result: Compared with the control group,the model group had no normal morphological structure of nerve cells and the number of nerve cells was significantly decreased,the organ coefficients of splenic organ and brain significantly decreased(P<0.05),Aβ was significantly increased(P<0.05).Compared with the model group,the BYHWT group recovered normal morphological structure of nerve cells and the number of nerve cells was significantly increased,the organ coefficients of splenic organ and brain significantly increased(P<0.05),Aβ was significantly decreased(P<0.05). Conclusion: BYHWT can improve normal morphological structure of nerve cells of Alzheimer's disease mice,increase organ coefficients of splenic organ and brain and decrease Aβ responses.