玛咖对运动训练大鼠睾酮及相关激素和抗疲劳能力的影响

Effect of Lepidium meyennii on Testosterone Content, Correlated Hormones Content and Exercise Capacity in Rats Receiving Exercise Training

摘要:

目的: 研究玛咖对运动训练大鼠睾酮及相关激素含量和抗疲劳能力的影响. 方法: 以大强度耐力训练大鼠为模型,55只42 d龄雄性Wistar大鼠为对象,随机分为5组:静止组、运动组、运动+低剂量玛咖组,运动+中剂量玛咖组,运动+高剂量玛咖组,每组10只(剔除不符合实验要求的大鼠5只).每天灌胃(ig)1次,玛咖组剂量分别为0.2,0.4,1.2 g·kg^-1,灌胃体积5 mL·kg^-1,静止组,运动组ig等量生理盐水.42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮等生化指标. 结果: 体重,运动组小于静止组(P<0.05);玛咖各组大于运动组(P<0.05),玛珈各剂量组间无显著差异.力竭游泳时间,运动组与静止组无明显差异;玛咖各组明显长于运动组(P<0.01),且随剂量增大而延长.血清睾酮水平,运动组低于静止组(P<0.01);玛咖各组分别为(4.78±1.58),(4.95±1.54),(5.06±1.59) nmol·L^-1,高于运动组(P<0.01),且玛咖各组组间无显著差异.血清皮质酮水平,各组间均无显著差异.血清睾酮与皮质酮比值变化与睾酮变化较为一致.血清促黄体生成素、促卵泡激素水平,静止组高于运动组,但无显著差异;玛咖各组高于运动组(P<0.01),且玛咖各组组间无显著差异. 结论: 补充玛咖可以纠正由于运动导致的下丘脑-垂体-性腺轴功能的紊乱,有效预防血清睾酮水平的降低,进而增强抗疲劳能力.

Abstract:

Objective: To study the effects of Lepidium meyennii on the content of testosterone, correlated hormones and anti-fatigue ability of rats after exercise. Method: By using the model of high-intensity endurance training, fifty-five 6-week-old male Wistar rats were randomly divided into 5 groups, with 10 in each group (5 rats which did not meet the requirement were removed):still in control group (C group), motion control group (M group), exercise+ig low-dose L. meyennii group (OML group), exercise+ig middle-dose L. meyennii group (OMM group), and exercise+ig high-dose L. meyennii group (OMH group). Gavage was performed using professional device once a day. The rats in L. meyennii groups were gavaged with 0.2, 0.4, 1.2 g· kg^-1 with ig volume of 5 mL· kg^-1. The rats in C and M groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: Body weight of the rats in M groups was lower than those in C group (P<0.05), and those in all doses of L. meyennii groups were higher than in M group (P<0.05) which did not show any differences among groups. Swimming time in all doses of L. meyennii groups were longer than in M group (P<0.01), and there were dose-response. But there were no differences between C and M groups. Serum testosterone in M group was lower than C group (P<0.01), in the same time, serum testosterone were higher in all doses of L. meyennii groups than M group[OML:(4.78±1.58) nmol· L^-1;OMM:(4.95±1.54) nmol· L^-1;OMH:(5.06±1.59) nmol· L^-1, P<0.01]. The serum corticosterone levels in each group showed no significant differences. Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. There were no differences in luteinizing hormone and follicle-stimulating hormone among C group and M group. But luteinizing hormone in all doses of L. meyennii groups were higher than in M group (P<0.01). In the mean time, follicle-stimulating hormone in all doses of L. meyennii groups were higher than in M group (P<0.01). There were no differences in both luteinizing hormone and follicle-stimulating hormone between groups in all doses of L. meyennii groups. Conclusion: The supplement of L. meyennii can alleviate the hypothalamus-pituitary-gonad axis function disorder caused by exercise through multiple targets and multiple ways. Finally, L. meyennii can prevent the decrease of serum testosterone after high-intensity exercise and improve anti-fatigue ability.