五子承气汤对大鼠高尿酸血症的影响

Effects of Wuzi Chengqi Decoction on Hyperuricemia Rats

摘要:

目的: 研究五子承气汤对高尿酸血症大鼠的影响. 方法: 将雄性SD大鼠60只随机分为6组,即:正常组、模型组、五子承气汤高、中、低剂量组(104,52,26 g·kg^-1)、阳性对照苯溴马隆(10 mg·kg^-1)组,采用腺嘌呤(100 mg·kg^-1,ig,qd × 28 d)和氧嗪酸钾(250 mg·kg^-1,ip,1次/周,连续4周)制备高尿酸血症大鼠模型.造模4周后,检测各组大鼠尿尿酸(UA),血尿酸(UA),血黄嘌呤氧化酶(XOD),血肌酐(SCr),尿素氮(BUN). 结果: 与正常组相比,模型组大鼠的尿UA显著降低,血UA,XOD,Cre及BUN 明显升高,差异具有显著性(P<0.01);五子承气汤低、中、高剂量组尿UA分别为(146.3±22.5),(202.2±53.8),(218.1±62.6) μmol·L^-1,显著高于模型组(89.8±27.1)μmol·L^-1(P<0.01,P<0.05);血UA分别为(207.3±42.5),(177.1±50.5),(158.0±49.0)μmol·L^-1,显著低于模型组(285.0±54.1)μmol·L^-1(P<0.01,P<0.05);五子承气汤还显著降低高尿酸血症大鼠血清XOD活性,显著降低SCr,BUN含量( P<0.01,P<0.05). 结论: 五子承气汤明显促进尿UA排泄,降低血UA含量,抑制血清XOD活性,改善肾功能,对大鼠高尿酸血症有一定的防治作用.

Abstract:

Objective: To observe the effects of Wuzi Chengqi decoction on hyperuricemia rats. Method: Sixty SD male rats were divided randomly into 6 groups: normal control, model control, positive control, Wuzi Chengqi decoction high, middle and low dose groups(104, 52, 26 g· kg^-1). The hyperuricemia rat models induced by adenine(100 mg· kg^-1,ig,qd×28 d) and potassium oxonate (250 mg· kg^-1,ip,qw×4weeks). High, middle and low dose groups were given Wuzi Chengqi decoction 104, 52 and 26 g· kg^-1,ig respectively.After 4 weeks of modeling,the rats in each group of serum uric acid (UA),blood urea nitrogen (BUN),serum creatinine(SCr),xanthine oxidase (XOD),urinary UA and renal indices were examined. Result: Compared with normal group, UA in urine was decreaed, while UA, XOD,Cre and BUN in serum were increased significantly (P<0.01). The content of urine uric acid of low,middle and high dose groups were (146.3±22.5), (202.2±53.8),(218.1±62.6) μmol· L^-1 respectively,which was significantly higher than those of the model group (89.8±27.1)μmol· L^-1(P<0.01,P<0.05). The content of serum uric acid of low,middle and high dose groups were (207.3±42.5),(177.1±50.5),(158.0±49.0)μmol· L^-1 respectively,which was significantly lower than those of the model group(285.0±54.1)μmol· L^-1(P<0.01,P<0.05).Wuzi Chengqi decoction also could significantly inhibited the serum XOD activity,decrease the content of serum SCr and BUN(P<0.01,P<0.05). Conclusion: Wuzi Chengqi decoction has good preventive and therapeutic effect on hyperuricemia rats induced by adenine and potassium oxonate.