加味独活寄生汤对胶原诱导性关节炎大鼠关节滑膜及血清中炎症因子的影响

Effects of Jiawei Duhuo Jisheng Tang on Expression Levels of Inflammatory Factors in Knee Joint Synovial and Serum of Type Ⅱ Collagen-induced Arthritis in Rats

摘要:

目的: 观察加味独活寄生汤对Ⅱ型胶原诱导性关节炎(CIA)大鼠IL-6,IL-17及TNF-α表达的影响. 方法: 取雄性SD大鼠90只,正常组6只,其余大鼠尾根部按0.2 mg/只肌肉注射胶原乳剂制成关节炎大鼠模型,选择造模成功大鼠30只,随机等分为模型组,来氟米特组和加味独活寄生汤高、中、低剂量组.来氟米特组按10 mL·kg^-1灌胃给予来氟米特,加味独活寄生汤高、中、低剂量组分别按44,22,11 g·kg^-1灌胃给予加味独活寄生汤,模型组及正常组灌胃相同体积水,每天1次,连续12周.采用RT-PCR法检测关节滑膜IL-6,IL-17,TNF-α的mRNA表达,酶联免疫吸附试验(ELISA)法检测血清IL-6,IL-17,TNF-α的质量浓度.统计方法采用单因素方差分析. 结果: 与正常组比较,模型组IL-6,IL-17,TNF-α的mRNA及蛋白表达显著升高;与模型组比较,各治疗组IL-6,TNF-α的mRNA及蛋白表达显著降低;加味独活寄生汤组IL-17蛋白表达显著降低,基因表达无显著性差异. 结论: 加味独活寄生汤对CIA大鼠关节炎症具有明显改善作用,其抗炎作用机制可能是通过下调炎性细胞因子IL-6,IL-17,TNF-α的表达来实现.

Abstract:

Objective: To observe effects of Jiawei Duhuo Jisheng Tang on mRNA transcription expression levels of interleukin-17(IL-17),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) in knee joint synovial and protein expression in serum of type Ⅱ collagen-induced arthritis in rats. Method: A rat model of collagen induced arthritis(CIA) was developed with male SPF sprague-dawley rats,thirty CIA rats were divided randomly into 5 groups,the model group were given distilled water(ig,10 mL· kg^-1),the leflunomide group(ig,10 mL· kg^-1),low,medium and high dosage groups of Jiawei Duhuo Jisheng Tang(ig,11,22,44 g· kg^-1),6 rats each group.Rats in the normal group were given the same dosage of distilled water,all rats received oral administration of corresponding medicines once daily for 12 weeks.Levels of interleukin-6(IL-6),interleukin-17(IL-17) and tumor necrosis factor-α(TNF-α) in serum were examined by ELISA,mRNA transcription expression levels of IL-6,IL-17,TNF-α were detected by RT-PCR.Statistical methods was employed by variance analysis. Result: Compared with the normal group,mRNA and protein expression of IL-6,IL-17,TNF-α increased significantly in the model group. Compared with the model group,mRNA and protein of IL-6,TNF-α in treatment groups was down-regulated,protein expression of IL-17 of Jiawei Duhuo Jisheng Tang decreased significantly and mRNA expression of IL-17 was no significant differences. Conclusion: Jiawei Duhuo Jisheng Tang shows a certain therapeutic effect on CIA rats,its anti-inflammatory action mechanism may achieve by reducing expression of IL-6,IL-17 and TNF-α.