HPLC同时测定山腊梅清感茶中4种黄酮苷的含量

Simultaneous Determination Content of Four Flavonoid Glycosides in Shanlamei Qinggan Cha by HPLC

摘要:

目的: 建立高效液相色谱方法同时测定山腊梅清感茶中4种黄酮苷含量的方法. 方法: 采用依利特hypersil ODS2色谱柱(4.6 mm× 250 mm,5 μm),以乙腈-0.1%冰醋酸为流动相进行梯度洗脱,流速1.0 mL·min^-1,检测波长344 nm,柱温35 ℃. 结果: 芦丁在7.012~70.12 mg·L^-1线性关系良好(r=0.999 6),平均回收率为100.68%,RSD 3.3%,山柰酚-3-O-β-D-半乳糖-(6-1)-α-L-鼠李糖苷在5.126~51.26 mg·L^-1线性关系良好(r=0.999 9),平均回收率为98.48%,RSD 1.7%,山柰酚-3-O-芸香糖苷在2.631~26.31 mg·L^-1线性关系良好(r=0.999 8),平均回收率为101.82%,RSD 1.9%,紫云英苷在2.634~26.34 mg·L^-1线性关系良好(r=0.999 9),平均回收率为97.02%,RSD 1.7%. 结论: 该方法操作简便,准确,重复性好,可用于山腊梅清感茶的质量控制.

Abstract:

Objective: The aim of this study was to establish an HPLC method for simultaneous determination of the content of four flavonoid glycosides in Shanlamei Qinggan Cha. Method: Using an Elite hypersil ODS2 column(4.6 mm×250 mm,5 μm), the mobile phase was acetonitrile-0.1% acetic acid, with flow rate of 1.0 mL·min^-1. The detective wavelength was at 344 nm and the column temperature was 35 ℃. Result: The standard curve presented a linear range from 7.012-70.12 mg·L^-1 for rutin(r=0.999 6),the average recovery was 100.68% with RSD of 3.27%.The calibration curve was linear in the range of 5.126-51.26 mg·L^-1 for kaempferol-3-O-β-D-galactose -(6-1)- α-L-rhamnoside (r=0.999 9), the average recovery was 98.48% with RSD of 1.7%. The calibration curve was linear in the range of 2.631-26.31 mg·L^-1 for kaempferol-3-O-rutinoside (r=0.999 8), the average recovery was 101.82% with RSD of 1.9%. The standard curve presented a linear range from 2.634-26.34 mg·L^-1 for astragalin (r=0.999 9), the average recovery was 97.02% with RSD of 1.7%. Conclusion: The method was fast, accurate and simple in good reproducibility, which could be used to control the quality of Shanlamei Qinggan Cha.