UPLC测定银翘散中的多指标性成分

Determination of Multiple Index Components in Yinqiao Powder by UPLC

摘要:

目的: 建立UPLC同时测定银翘散中绿原酸、甘草苷、橙皮苷、牛蒡子苷、连翘苷含量的方法. 方法: 采用UPLC系统,ACQUITY UPLC BEH Shield RP18色谱柱(2.1 mm×50 mm,1.7 μm),甲醇-0.15%甲酸溶液梯度洗脱,流速0.25 mL·min^-1,检测波长279 nm,柱温40 ℃,同时测量5种活性成分的含量. 结果: 绿原酸、连翘苷、牛蒡子苷、橙皮苷、甘草苷分别在0.018 76~0.043 77,0.002 37~0.011 85,0.011 21~0.056 06,0.001 34~0.006 71,0.001 71~0.012 38 μg线性关系良好(r≥0.999 1),平均回收率分别为97.12%(RSD 0.9%),101.40%(RSD 2.1%),100.61%(RSD 1.6%),100.79%(RSD 0.7%),101.04% (RSD 1.7%). 结论: 该方法简便、快速、专属性强,可用于银翘散的质量控制.

Abstract:

Objective: The aim of this study was to establish a method for the content determination of chlorogenic acid, liquiritin, hesperidin, arctiin and phillyrin in Yinqiao Powder. Method: UPLC system was performed on ACQUITY UPLC BEH Shield RP₁₈ column(2.1 mm×50 mm,1.7 μm)at 40 ℃. The mobile phase was consisted of methanol-0.15% formic acid solution in gradient elution mode. The flow rate was set at 0.25 mL·min^-1 and the detection wavelength was set at 279 nm. Result: Chlorogenic acid, phillyrin, arctiin, hesperidin and liquiritin had good linear relationship in the ranges of 0.018 76-0.043 77, 0.002 370-0.011 85, 0.011 21-0.056 06, 0.001 34-0.006 71 and 0.001 71-0.012 38 μg, respectively. The corre-lation coefficients were all more than 0.999 1.The average recoveries were 97.12%(RSD 0.9%), 101.40%(RSD 2.1%), 100.61%(RSD 1.6%), 100.79%(RSD 0.7%)and 101.04%(RSD 1.7%), respectively. Conclusion: The method is simple, rapid, specific, and can be used for quality control of Yinqiao powder.