提取溶剂对柴黄颗粒中主要成分的影响

Comparison on Effective Components of Chaihuang Granules Among Three Diffrernt Extracting Solvents

摘要:

目的: 比较不同提取溶剂对柴黄颗粒HPLC图谱及对主要成分色谱峰面积的影响,为改进和完善柴黄颗粒的质量标准提供依据. 方法: 以HPLC对照品比对法对主要色谱峰进行归属;以检出色谱峰的相对峰面积、色谱峰面积总量,以及主要成分的相对比值及总量对水、甲醇以及70%甲醇等3种提取溶剂的HPLC图谱进行比较分析. 结果: 共指认了黄芩苷、汉黄芩苷、黄芩素、汉黄芩素等4个色谱峰,3种提取溶剂的HPLC图谱基本一致,但主要色谱峰的峰面积及总峰面积有较大差异,黄芩苷等4种主要成分的相对比值及总量也有较大差异.在建立的色谱条件下,3种提取溶剂的成分含量有明显差异,以水为提取溶剂主要成分含量最高. 结论: 本研究为改进柴黄颗粒的含量测定方法提供了有益的参考.

Abstract:

Objective: To compare the differences of HPLC profile and the main components among 3 different solvents so as to provide the basis for improving the quality standard of Chai-huang granules. Method: HPLC was employed to identify the main chromatographic peaks, which also was taken to compare the relative peak amounts, total amounts as well as the relative ratio among water, methanol and 70% methanol solvent extracts. Result: Four peaks (baicalin, wogonoside, baicalein, wogonin) were identified. HPLC profiles of 3 extract solvents were almost same, however there were exhibited the significant varieties of the peak amounts, total amounts and the relative ratio of 4 major components. The contents of 3 extract solvents had substantial differences, and water as extracting solvent was highest. Conclusion: This study could offer the useful reference for determination method of Chai-huang granules.