GC测定降香油中氧化橙花叔醇Ⅰ、氧化橙花叔醇Ⅱ、反式橙花叔醇的含量

GC Determination Oxidation Nerolidol Ⅰ, Oxidation Nerolidol Ⅱ, Trans Nerolidol of Content in Dalbergiae Odoriferae Oil

摘要:

目的: 建立降香油中氧化橙花叔醇Ⅰ、氧化橙花叔醇Ⅱ、反式橙花叔醇含量测定方法,并比较不同批号的降香油中氧化橙花叔醇Ⅰ、氧化橙花叔醇Ⅱ、反式橙花叔醇的含量. 方法: 采用气相色谱法,DB-Wax弹性石英毛细管柱(250 μm×30 m,0.25 μm),以FID检测器,内标为萘. 结果: 降香油中氧化橙花叔醇Ⅰ、氧化橙花叔醇Ⅱ、反式橙花叔醇的含量分别为16%~20%,19%~25%,25%~30%,氧化橙花叔醇Ⅰ、氧化橙花叔醇Ⅱ、反式橙花叔醇分别在0.018 96~0.113 73,0.019 01~0.114 06,0.013 02~0.078 09 g·L^-1呈良好的线性关系,平均回收率分别为98.10%,98.79%,98.95%. 结论: 采用气相色谱法测定降香油中氧化橙花叔醇Ⅰ、氧化橙花叔醇Ⅱ、反式橙花叔醇含量,方法简单、可行.7个批号的降香油的含量差别不大.

Abstract:

Objective: To establish the determination method of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol,and to compare the content of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol in different batches of dalbergiae odoriferae oil. Method: Gas chromatography, DB-Wax fused silica capillary column (250 μm × 30 m, 0.25 μm), with FID detector, internal standard was naphthalene. Result:The content of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol in dalbergiae odoriferae oil were 19% to 25%, 16% to 20%, 25% to 30%. Oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol were good linear separately within the concentration range of 0.030 4-0.114, 0.030 4-0.114, 0.020 8-0.078 g·L^-1. The average recoveries were 99.27%, 99.41%, 98.68%. Conclusion: The GC method was used to determine the content of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol in dalbergiae odoriferae oil is simple and feasible.There is no apparent difference in the content of seven batches of the dalbergiae odoriferae oil.