HPLC测定苏黄止咳胶囊中紫苏醛含量

Determination of Perillaldehyde in Suhuang Zhike Capsule by High-performance Liquid Chromatography

摘要:

目的: 建立苏黄止咳胶囊中紫苏醛含量的HPLC测定方法. 方法: 采用高效液相色谱法,SHIMADZU Shim-pack VP-ODS C₁₈色谱柱 (4.6 mm × 250 mm, 5 μm),流动相甲醇-水(68:32),检测波长230 nm,流速0.8 mL·min^-1,柱温30 ℃. 结果: 紫苏醛在5.672~453.76 ng与其峰面积呈良好的线性关系,线性回归方程为Y=5.695X+1.28(r=0.999 5),平均加样回收率为99.57% (RSD 1.7%). 结论: 该方法准确、分离度好、专属性强,可用于苏黄止咳胶囊中紫苏醛的含量测定.

Abstract:

Objective: To establish a method for the content determination of perillaldehyde in Suhuang Zhike Capsule. Method: HPLC was performed on SHIMADZU Shim-pack VP-ODS C₁₈ column (4.6 mm × 250 mm, 5 μm), and the mobile phase was consisted of methanol-water (68:32). The detection wavelength was set at 230 nm, the flow rate of 1.0 mL·min^-1 and the column temperature was 30 ℃. Result: Perillaldehyde showed a good linear relationship from 5.672 ng to 453.76 ng, and the regression equation was Y=5.695X+1.28 (r=0.999 5). The average recovery was 99.57 % with RSD of 1.68 %. Conclusion: The HPLC method is simply and accurate with a good specificity. It can be used to determine the content of perillaldehyde in the Suhuang Zhike Capsule.