鲜、干马齿苋药材中生物碱类成分的含量差异

Content Difference of Total Alkaloids in Fresh and Dried Portulacae Herba

摘要:

目的: 比较鲜、干药材中去甲肾上腺素和多巴胺的含量差异. 方法: 采用HPLC-UV测定马齿苋中去甲肾上腺素和多巴胺含量,流动相甲醇-水[70:30,含30 mmol·L^-1十二烷基硫酸钠和0.02 mol·L^-1磷酸盐缓冲液(pH 3.2)],流速0.8 mL·min^-1,检测波长280 nm. 结果: HPLC中去甲肾上腺素和多巴胺的分离度很好,二者线性范围分别为52.5~157.5,64.7~194.1 mg·L^-1.鲜马齿苋中去甲肾上腺素和多巴胺质量分数分别为0.070%和0.50%,二者在干品中质量分数分别为0.015%和0.20%. 结论: 鲜药材中去甲肾上腺素和多巴胺含量明显高于干药材,可为马齿苋的合理使用提供实验依据.

Abstract:

Objective: To compare contents of norepinephrine and dopamine in fresh and dry Portulacae Herba. Method: HPLC-UV method was adopted for determination of norepinephrine and dopamine in fresh and dry Portulacae Herba.This analysis was performed on a Alltima C₁₈ column(4.6 mm×250 mm,5 μm),column temperature was set at 25 ℃,flow rate was 0.8 mL·min^-1,detection wavelength was kept at 280 nm, mobile phase consisted of methanol-water(70:30,containing 30 mmol·L^-1 sodium dodecyl sulfate and 0.02 mol·L^-1 phosphate buffer with pH of 3.2). Result: Separation of norepinephrine and dopamine was well in HPLC,these two alkaloids showed a good linear relationship in 52.5-157.5 mg·L^-1 and 64.7-194.1 mg·L^-1,respectively.Contents of norepinephrine and dopamine in fresh Portulacae Herba were 0.070% and 0.50%;while contents of them in dry Portulacae Herba were 0.015% and 0.20%,respectively. Conclusion: Contents of norepinephrine and dopaminein in fresh herbs are significantly higher than these in dry herbs.This paper provides experimental basis for reasonable application of Portulacae Herba.