UPLC测定大鼠血浆中士的宁的含量

Determination of Strychnine in Rat Plasma by UPLC

摘要:

目的: 建立能测定大鼠血浆中通脉丸浓缩液中士的宁含量的UPLC分析方法. 方法: 大鼠血浆样品以液液萃取法处理后用BEH C₁₈色谱柱分离,流动相乙腈-酸水(23:77),酸水为0.02 mol·L^-1磷酸二氢钾与0.01 mol·L^-1庚烷磺酸钠等量混合,并用10%磷酸调体系pH 2.5;流速0.18 mL·min^-1,检测波长260 nm,柱温30 ℃. 结果: 血浆中内源性物质不干扰士的宁的测定,士的宁在0.043 2~10.8 mg·L^-1线性良好,日内、日间精密度均<10%,平均回收率均>85%,稳定性符合体内药物分析要求. 结论: 该方法灵敏度高、紧密度好,可应用于通脉丸浓缩液中士的宁的药物动力学研究.

Abstract:

Objective: To establish an effective method for determining the strychnine of Tongmai pill concentrate (TPC) in rat plasma by UPLC. Method: The rat plasma samples were processed by liquid-liquid extraction and separated on a BEH C₁₈ column. The mobile phase consisted of 23% acetonitrile and 77% water containg 0.02 mol·L^-1 potassium dihydrogen phosphate and 0.01 mol·L^-1 sodium heptanes sulfonate (adjusting pH 2.5 with 10% phosphonic acid.The flow rate was 0.18 mL·min^-1. The detection wavelength was set at 260 nm and the column temperature was 30 ℃. Result: The determination of strychnine was not interferred by the endogenous in the rat plasma. The calibration curve of strychnine was in good linearity within the range of 0.043 2-10.8 mg·L^-1. The mean extraction recoveries were more than 85% and intra-and inter-day precisions were less than 10%. The stability results meet the requirements for the biopharmaceutical analysis. Conclusion: This sensitivite and precise analytical method is applicable to the pharmacokinetic study of strychnine of TPC in rat plasmas.