舒胃汤对功能性消化不良大鼠胃肠动力及ENS-ICC-SMC网络超微结构的影响

Effect of Shuwei Decoction on Gastrointestinal Motility and Ultratructure of ENS-ICC-SMC Network in Rat with Functional Dyspepsia

摘要:

目的: 观察舒胃汤对功能性消化不良(functional dyspepsia,FD) 肝郁脾虚型大鼠胃肠动力及胃肠道神经-Cajal间质细胞-平滑肌(ENS-ICC-SMC)网络超微结构的影响,探讨舒胃汤治疗 FD 的机制. 方法: 将72只大鼠随机分为正常组、模型组、舒胃汤低剂量组(舒低组)、舒胃汤中剂量组(舒中组) 和舒胃汤高剂量组(舒高组),莫沙比利组(西药组),每组 12只.舒低、中、高分别给予舒胃汤 7.67,15.34,30.68 g·kg^-1,莫沙必利组予莫沙必利 1.37 mg·kg^-1.采用复合病因造模(慢性束缚应激+过度疲劳+饮食失节),造成FD肝郁脾虚证大鼠模型.造模后第3天各组给予相应药液,对照组和模型组每日予以蒸馏水(10 mL·kg^-1),均为每日1次,持续14 d.检测大鼠胃内残留率,小肠推进率,电镜下观察大鼠胃窦部和十二指肠组织ENS-ICC-SMC网络超微结构. 结果: 与对照组比较,模型组胃排空延迟,胃内残留率升高,小肠推进率下降(P<0.05).与模型组比较,舒高组、舒中组、莫沙必利组胃内残留率明显降低,小肠推进率明显升高(P<0.01).与模型组比较,舒高组、舒中组、莫沙必利组ICC细胞形态改善,ICC周围神经末梢较丰富,ICC相互之间及ICC与平滑肌细胞之间联系紧密. 结论: 舒胃汤能够改善ENS-ICC-SMC网络超微结构之间的相互联系,改善胃肠动力,从而有效治疗FD.

Abstract:

Objective: To observe the effect and mechanism of Shuwei decoction on gastrointestinal motility and gastrointestinal nerve-interstitial cells of Cajal-smooth muscle (ENS-ICC-SMC) network ultrastructure,to investigate in functional dyspepsia (functional dyspepsia, FD) with liver and spleen deficiency rats. Method: Seventy-two rats were randomly divided into normal group, model group,the low, middle and high dose groups of Shuwei decoction (7.67, 15.34, 30.68 g·kg^-1), mosapride group (1.37 mg·kg^-1),12 rats in each group.The model was made bychronic restraint stress combined with fatigue and improper diet. Three day after modeling corresponding drug,were give once daily for 14 days.The residual rate of stomach, small intestine propulsion rate were detected and to the structure of ENS-ICC-SMC network of gastric antrum and duodenum tissue were observed under electron microscope. Result: Compared with the control group, model group delayed gastric emptying, increased the gastric emptying rate, intestinal propulsion rate(P<0.05). Compared with the model group, treatment groups had significantly lower gastric emptying rate,and increased intestinal propulsion rate significantly (P<0.01). Compared with the model group, treatment groups improved ICC cells, ICC peripheral nerve endand relationship between ICC and ICC and between smooth muscle cells. Conclusion: Shuwei decoction can improve the relationship between the ultrastructure of ENS-ICC-SMC network, and improve the gastrointestinal motility, which is effective in the treatment of FD.