柴胡疏肝散对免疫损伤性肝纤维化的防治作用

Inhibitory Effects of Chaihu Shugan San on Hepatic Fibrosis in Rats

摘要:

目的: 探讨柴胡疏肝散对肝纤维化的防治作用. 方法: 70只Wistar大鼠随机分为7组:正常组、病理模型组、秋水仙碱治疗组、柴胡疏肝散低、中、高剂量组、柴胡疏肝散预防组.除正常组外,其余各组均采用猪血清腹腔注射诱发肝纤维化,0.5 mL/只,2次/周,连续10周,5周后即可形成肝纤维化.预防组于造模同时给药(以柴胡疏肝散6.3 g·kg^-1),各治疗组于造模第6周给药,持续至第10周.秋水仙碱治疗组剂量0.5 mL·kg^-1·d^-1,柴胡疏肝散低、中、高剂量组(3.5,6.3,12.6 g·kg^-1·d^-1).用全自动分析法测定各组血清肝功能、放射免疫法测定肝纤维化指标,采用酸性水解法检测肝组织羟脯氨酸(hydroxyproline,HYP)含量,并观察病理变化. 结果: 与模型组比较,柴胡疏肝散中剂量组血清丙氨酸转氨酶(ALT),(156.5±3.2) U·L^-1 VS (78.4±4.6) U·L^-1(P<0.01);天冬氨酸转氨酶(AST),(387.4±12.7) U·L^-1 VS (232.5±13.5) U·L^-1(P<0.01);r-谷氨酰转肽酶(GGT),(128.2±4.0) U·L^-1 VS (78.5±4.2) U·L^-1(P<0.01);碱性磷酸酶(ALP),(152.75±5.01) U·L^-1 VS(42.51±4.67) U·L^-1(P<0.01);透明质酸(HA),(337.2±22.1)VS(140.6±21.2) μg·L^-1(P<0.01);PCⅢ(145.31±17.14) μg·L^-1VS(85.5±19.7)μg·L^-1(P<0.05);Ⅳ型胶原(135.1±22.4)VS(88.37±21.09)(P<0.05);层黏连蛋白(LN),(317.5±21.3)VS(148.4±18.4),(P<0.05)含量均显著下降, 肝组织(HYP)(527.2±34.2)VS(346.2±43.1)(P<0.01)含量显著降低. 结论: 柴胡疏肝散有明显的抗肝纤维化作用.

Abstract:

Objective: To investigate the protective effects and its mechanism of Chaihu Shugan San(CHSGS) on hepatic fibrosis in rats. Method: The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum to rats.Seventy Wistar rats were divided into seven groups:blank group, model group, CHSGS high-dose group, mediate-dose group and low-dose group, prevention group, colchicin contrast group. Except the black groups, the other groups were injected with pig's serum without inactivation into abdominal cavity, twice per week and 0.5 mL each time, for 10 weeks. The prevention group was injected the stomach with the medicines(CHSGS 6.3 g·kg^-1·d^-1,10 weeks), the other treatment groups were treated as the prevention groups 6 weeks after modeling for 4 weeks. Then rats were randomly taken for decollation in six groups at the end of the 10th week:colchicin contrast group 0.5 mL·kg^-1·d^-1,CHSGS low-dose, mediate-dose and high-dose groups (3.5,6.3,12.6 g·kg^-1·d^-1).The changes of the serum biochemical indicator of the hepatic function and hepatic fibrosis were detected respectively. Result: Compared with model group,the serum content of alanine aminotransferase(ALT),aspartate aminotransferase (AST),r-glutamyl endopeptidase(GGT), alkaline phosphatase(ALP) and typeⅠ,Ⅲ,Ⅳcollage, hyaluronic acid (HA), laminin (LN) were depressed obviously in treatment groips,the content of hydroxyproline(HYP) in liver tissue was significantly lower in CHSGS mediate-dose group. Conclusion: Chaihu Shugan San has an effect on hepatic fibrosis model rats.