爱罗咳喘宁对COPD大鼠肺组织炎症因子及氧化应激的影响

Effect of Ailuo Kechuanning on Inflammatory Cytokines and Oxidative Stress of Lung Homogenates in Rats with COPD

摘要:

目的: 探讨爱罗咳喘宁方对慢性阻塞性肺疾病(COPD)肺部炎症及氧化损伤作用及机制. 方法: 采用脂多糖(LPS)加烟雾诱导 COPD大鼠模型,大鼠随机分为正常组、模型组、爱罗咳喘宁低、中、高剂量组.正常组、模型组灌胃生理盐水(15.52 mL·kg^-1·d^-1), 爱罗咳喘宁低、中、高剂量组分别灌胃(7.75,15.52,31.04) g·kg^-1·d^-1,连续14 d.用比色法、酶联免疫法(ELISA)分别测定大鼠肺匀浆中磷酸化p38MAPK(mitogen activatein kinase,p-p38MAPK)、髓过氧化物酶(MPO)活性,白介素-17(IL-17)和C反应蛋白(CRP)含量;并测定肺匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量. 结果: 与正常组比较,模型组p-p38MAPK和MPO活性、IL-17和CRP含量均显著升高(均P<0.01);SOD和GSH-Px活性均显著降低、MDA含量显著升高(均P<0.01);与模型组相比,爱罗咳喘宁中剂量组p-p38MAPK(P<0.05)、MPO(P<0.01)活性与IL-17,CRP(均P<0.01)含量均显著降低,SOD和GSH-Px活性均显著升高、MDA含量显著降低(均P<0.01). 结论: 爱罗咳喘宁对COPD有抗炎作用,其机制可能与抑制p-p38MAPK,MPO活性,减少IL-17的释放有关;爱罗咳喘宁对COPD有抗抗氧化损伤作用,其机制通过提高SOD和GSH-Px活性,减少MDA生成,保护肺组织的结构与功能.

Abstract:

Objective: To study the effect of Ailuo Kechuanning on mitogen activatein kinase(p38MAPK),myeloperoxidase(MPO),interleukin-17(IL-17),C-reactive protein(CRP),and oxidative stress in rats with chronic obstructive pulmonary disease (COPD). Method: Lipopolysaccharide(LPS) smoke wasused to COPD rat model,the experimental animals were randomly divided into normal group,model group,Ailuo Kechuanning groups(7.75,15.52,31.04 g·kg^-1·d^-1),The activity of p-p38MAPK and MPO was detected by colorimetric method,enzyme-linked immunosorbent assay was used to determine the change of IL-17 and C-reactive protein(CRP) levels.the concentration of MDA, the activity of SOD and glutethione peroxiadase(GSH-Px) were measured in lung homogenates of all the groups. Result: The activity of p-p38MAPK and MPO, the levels of IL-17 and CRP were higher significantly,the activity of SOD and GSH-Px were decreased, the level of MDA was higher significantly in lung homogenates of the model group. Compared with the normal control group. While the activity of p-p38MAPK and MPO, and the levels of IL-17 and CRP was decreased significantly.the activity of SOD and GSH-Px were higher significantly, and the level of MDA was decreased significantly in lung homogenates of the rats treated with Ailuo Kechuanning(15.52 g·kg^-1·d^-1)compared with the model group. Conclusion: Ailuo Kechuanning may improve the pulmonary function of COPD. The effects of Ailuo Kechuanning for treatment of COPD may inhibite the activity of p-p38MAPK and MPO,and decrease the levels of IL-17, CRP and MDA, lead to the anti-inflammatory and against oxidative damage.