西洋参茎叶皂苷抗大鼠心肌缺血再灌注损伤的作用及机制

Protective Effects and Mechanism of Panax quinquefolius Saponins on Myocardial Ischemia of Rats

摘要:

目的: 观察西洋参茎叶皂苷(Panax quinquefolius saponins,PQS)对大鼠心肌缺血再灌注损伤的保护作用,并探讨其作用机制. 方法: 大鼠50只随机分5组,每组10只,即假手术组、模型组、PQS低、高剂量组及尼莫地平组.假手术组和模型组ig给予生理盐水,给药组ig给予PQS(100,300 mg·kg^-1)和尼莫地平(21.6 mg·kg^-1),每日1次,连续给药15 d后,除假手术组外,手术结扎冠脉建立心肌缺血再灌注模型.观察PQS对大鼠心脏功能及心肌梗死面积的影响;检测PQS对血清肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及心肌组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平的影响. 结果: 模型组左室收缩压(LVSP)降低,左室舒张末压(LVEDP)升高,心肌梗死面积增加,血清中CPK,LDH活性及MDA,TNF-α和IL-6含量升高,SOD和GSH-Px活性降低,与假手术组比较均具有显著统计学差异(P<0.01);与模型组比较,PQS和尼莫地平均可以使LVSP升高,LVEDP降低,减少心肌梗死面积,降低血清中CPK,LDH活性及MDA,TNF-α和IL-6含量,升高SOD和GSH-Px活性,均具有统计学差异(P<0.01,P<0.05). 结论: PQS预处理能够有效的减弱心肌缺血再灌注引起的损伤,该作用与抑制心肌缺血再灌注引起的活性氧增加及减少炎症反应的发生有关.

Abstract:

Objective: To explore the protective effects of Panax quinquefolius saponins (PQS) and to investigate its possible mechanism on myocardial ischemia-reperfusion model of rats. Method: Fifty rats were randomly divided into 5 groups:sham operation group, model group, low-and high-dose PQS groups (100, 300 mg·kg^-1), and nimodipine group (21.6 mg·kg^-1) of 10 rats each. The myocardial ischemia-reperfusion model was induced by ligature of coronary artery and reperfusion of rats except for rats in sham operation goup. All rats were ig administrated with corresponding medicines once daily for 15 days. The heart function and cardiac infarction area were observed. The contents of creatine phosphokinase (CPK), lactic dehydrogenase (LDH), tumor necrosis factor (TNF) -α and interleukin (IL) -6 in serum and superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and malonaldehyde (MDA) in cardiac muscle tissue were tested. Result: Compared with sham operation group, the left ventricular systolic pressure was increased, the left ventricular end-diastolic pressure was decreased, cardiac infarction area was increased, and the contents of CPK, LDH, MDA, TNF-α and IL-6 were decreased, the activities of SOD and GSH-Px were inhibited in model group (P<0.01,P<0.05). The above indexes were improved in the PQS groups (P<0.01, P<0.05). Conclusion: PQS can attenuate the damage caused by myocardial ischemia reperfusion and its mechanism may be involved with the anti-oxidation and anti-inflammatory activities.