通便颗粒调节慢传输型便秘大鼠结肠水通道蛋白3,水通道蛋白8表达的研究

Experimental Study of Tongbian Granules on the Regulation of AQP3 and AQP8 Expression in Colon of Rats with Slow Transit Constipation

摘要:

目的: 观察通便颗粒对慢传输型便秘(STC)大鼠结肠水通道蛋白3(AQP3),水通道蛋白8(AQP8)的影响,阐明其治疗慢传输型便秘的作用机制. 方法: 选取健康SD大鼠60只,随机选取10只大鼠作为正常组,剩余50只大鼠采用复方苯乙哌啶法诱导慢传输型便秘大鼠动物模型,造模组大鼠随机分为模型组、通便颗粒低、中、高剂量组(9.3,18.7,37.3 g·kg^-1)、聚乙二醇散剂组(2.1 g·kg^-1),给药组ig给予相应剂量,正常组和模型组给予同体积生理盐水,连续给药30 d.记录大鼠体质量、24 h大便质量,运用炭墨灌胃法检测大鼠肠道传输功能,并运用免疫组织化学技术检测结肠AQP3,AQP8的分布、表达及相对含量. 结果: 与正常组比较,大鼠24 h大便质量降低,肠管炭墨推进率降低,AQP3,AQP8的表达增加,均具有统计学差异(P<0.05);给予药物干预后,与模型组相比,治疗组各组大鼠24 h大便质量均有所增加,差异明显(P<0.05);通便颗粒中、高剂量组、聚乙二醇散剂组肠管炭墨推进率明显增加,差异有统计学意义(P<0.05);免疫组化结果显示通便颗粒中、高剂量组和聚乙二醇散剂组能不同程度减少AQP3,AQP8的表达,以通便颗粒高剂量组最为明显(P<0.05). 结论: 通便颗粒具有明显改善便秘大鼠的排便功能,可能通过下调AQP3,AQP8的表达从而调节水分的吸收和分泌来治疗STC.

Abstract:

Objective: To observe the effects of Tongbian granules on the regulation of aquaporin 3 (AQP3) and aquaporin 8 (AQP8) in rats with slow transit constipation (STC), and to clarify its possible mechanism. Method: A slow constipation rat mode was induced by compound phenylethyl piperidine. The 50 model rats were randomly divided into model group, low-, medium-and high-dose Tongbian granules groups (9.3, 18.7, 37.3 g·kg^-1), and polyethylene glycol powder group (2.1 g·kg^-1). The another 10 normal rats were assigned to normal control group. All rats were ig adiminstrated with the corresponding medicines for 30 days.The body weight, 24-hour stool weight, the carbon powder propulsion in the colon of rats were recorded and measured. The distribution and relative content of AQP3 and AQP8 in rat colon were detected. Result: Compared with model group, the body weight, 24-hour stool weight, the carbon powder propulsion rate in the colon were decreased in model group. The above indexes were improved in the Tongbian granules groups (P<0.05). Immunohistochemistry results showed that the number of AQP3 and AQP8 were remarkably decreased in all dose Tongbian granules groups and polyethylene glycol powder group. Tongbian granules group of high dose had the best results (P<0.05). Conclusion: Tongbian granules has excellent curative effects in improving constipation defecation function of STC rats, which may be involved with the regulation of absorption and secretion of moisture achieved by depressing AQP3 and AQP8 in rat colon.