胃元宁对功能性消化不良小鼠胃肠运动及胃肠激素分泌的影响

Effects of Weiyuanning on Gastrointestinal Motility and Gastrointestinal Hormones in Mice with Functional Dyspepsia

摘要:

目的: 建立功能性消化不良(functional dyspepsia,FD)小鼠模型,研究胃元宁对FD小鼠胃肠运动及胃肠激素分泌的影响. 方法: 昆明种小鼠随机分为6组为正常组,胃元宁汤剂高、中、低剂量组(90,45,22.5 g·kg^-1),模型组,多潘立酮组 (10 mg·kg^-1),除正常组外,其他小鼠采用不规律进食联合左旋精氨酸(L-arginine,L-Arg)ip构建FD小鼠模型,造模成功后ig给予相应药物7 d,检测小鼠体重、胃内残留率和肠推进率;观测胃组织病理学变化;利用酶联免疫吸附测定法(ELISA)检测血清胃泌素(gastrin,GAS),血浆胃动素(motilin,MTL)的含量变化. 结果: 与正常组相比,各造模组小鼠体重及小肠推进率均明显下降 (P<0.01),胃内残留率均显著升高 (P<0.01);经治疗后,与模型组相比,胃元宁各剂量组均能显著增加小鼠体重及小肠推进率,并显著升高血清GAS、血浆MTL含量(P<0.05,P<0.01),同时可明显降低胃内残留率(P<0.05,P<0.01).治疗前后,与正常组相比,各实验组胃组织病理学表现均无差异. 结论: 不规律进食联合左旋精氨酸腹腔注射可成功构建FD小鼠模型;胃元宁能够促进胃肠运动,显著改善小鼠功能性消化不良,其机制可能与其升高血清GAS和血浆MLT水平有关.

Abstract:

Objective: To establish mice model of functional dyspepsia (FD) and to investigate the effects of Weiyuanning on gastrointestinal motility and gastrointestinal hormones. Method: The mice were randomly divided into normal group, model group, low-, medium-and high-dose Weiyuanning groups (22.5, 45, 90 g·kg^-1), and domperidone group (10 mg·kg^-1). FD models were made by irregular food intake plus intraperitoneal injection with L-arginine in mice except for mice in normal group. All mice were ig administrated with corresponding medicines for 7 days. The body weights, the gastric remnant rate and small intestinal propulsion rate were measured. The gastric histopathological changes were observed. The contents of gastrin (GAS) and motilin (MTL) were detected by Elisa. Result: Compared with model group, the body weight, gastric emptying rate were decreased, the small intestinal propulsion rate was increased in model group (P<0.01). The above indexes were improved in the Weiyuanning groups (P<0.01). Meanwhile, the the contents of GAS in serum and MTL in plasma were increased in Weiyuanning groups (P<0.05, P<0.01). No significant changes were found in the improvement of gastric histopathological among all groups after the treatment. Conclusion: FD model could be successfully made by irregular food intake plus intraperitoneal injection with L-arginine. Weiyuanning has significant effect in improving indigestion in FD mice. Its mechanism may be related to elevating the levels of GAS in serum and MTL in plasma of mice.