黑果枸杞含片体内抗氧化作用

Study on Antioxidant Effect of Lycium ruthenicum Buccal Tablet in vivo

摘要:

目的: 研究黑果枸杞含片的体内抗氧化作用. 方法: 40 只小鼠随机分为4组:正常组、黑果枸杞含片低、高剂量组(0.08,0.16 mg·g^-1)和阳性药组(维生素C, 0.02 mg·g^-1).各组分别连续ig给药28 d后眼眶采血及取肝组织,试剂盒测定小鼠血清和肝脏中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活性. 结果: 与正常组比较,黑果枸杞含片低、高剂量组小鼠的体重没有明显变化,小鼠血清和肝组织中MDA分别为(5.97±2.53),(11.08±3.72) μmol·L^-1和(5.84±1.44),(10.79±2.06) μmol·L^-1与正常组(8.69±2.85) μmol·L^-1,(14.49±1.56) μmol·L^-1相比显著降低(P<0.05,P<0.01),T-AOC,SOD,GSH-Px和CAT 活性明显提高(P<0.05,P<0.01),尤其是高剂量组T-AOC,SOD,GSH-Px和CAT活性显著提高. 结论: 黑果枸杞含片具有显著的体内抗氧化活性.

Abstract:

Objective: To study the antioxidant effect of Lycium ruthenicum buccal tablet in mice. Method: Fourty mice were randomly divided into 4 group:normal group (normal saline) low-and high-dose L. ruthenicum buccal tablet groups (0.08, 0.16 mg·g^-1), and vitamin C group (0.02 mg·g^-1). The mice were sacrificed by intragastric administration 28 days later and the total antioxidant capacity (T-AOC), the contents of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase enzyme (GSH-Px) and catalase (CAT) in serum and liver were determined using the test kits. Result: Compared with normal group, no significant changes were found in the mice body weight, while the contents of MDA in serum and liver was significantly decreased are (5.97±2.53),(11.08±3.72) μmol·L^-1 and (5.84±1.44),(10.79±2.06) μmol·L^-1(P<0.05,P<0.01), the activities of T-AOC, SOD, GSH-Px and CAT were significantly increased (P<0.05 or P<0.01) in high-and low-dose L. ruthenicum buccal tablet groups. Conclusion: L. ruthenicum buccal tablet has significant antioxidant activity in vivo.