黑莓籽降血脂有效部位研究

Study on Effective Extracts of Rubus spp Seed for Reducing Blood Lipid

摘要:

目的: 研究黑莓籽石油醚部位、正丁醇部位及黑莓籽油对高脂血症小鼠的脂质代谢和脂质过氧化的影响. 方法: 120只小鼠随机分为12组:正常组,模型组,阳性对照组和石油醚部位高剂量组(800 mg·kg^-1),中剂量组(400 mg·kg^-1)和低剂量组(200 mg·kg^-1),正丁醇部位高剂量组(600 mg·kg^-1),中剂量组(300 mg·kg^-1)和低剂量组(150 mg·kg^-1),黑莓籽油高剂量组(1 000 mg·kg^-1),中剂量组(500 mg·kg^-1)和低剂量组(250 mg·kg^-1),采用灌胃高脂乳剂诱导高脂血症小鼠模型, 测定血清中甘油三脂(TG),总胆固醇(TC),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C),丙二醛(MDA)的含量,超氧化物歧化酶(SOD)活性及肝、肾、脾系数. 结果: 石油醚部位低剂量组能显著性的降低血清中TG,TC,LDL-C和MDA的含量及肝、脾系数,升高HDL-C的含量及SOD的活性(P<0.05);正丁醇部位中、低剂量组均能显著性的降低血清中TG,TC,LDL-C,MDA的含量及肝、脾系数,升高SOD的活性(P<0.05);黑莓籽油中剂量组均能显著性的降低血清中TG,TC,MDA的含量及肝、脾系数,升高HDL-C的含量及SOD的活性(P<0.05);油高剂量组均能显著性的降低血清中TG,TC,MDA的含量及肝、脾系数,升高SOD的活性(P<0.05);正丁醇部位高剂量组能显著性的降低血清中TG,MDA的含量及肝、脾系数,升高HDL-C的含量及SOD的活性(P<0.05);油低剂量组能显著性的降低血清中TG,TC,LDL-C的含量及肝、脾系数,升高HDL-C的含量(P<0.05);石油醚部位高剂量组(TG,TC)和中剂量组(TC,HDL-C)及肝脾系数)能显著性改变,其他各指标虽不具有显著性,但与模型组相比,具有一定的效果. 结论: 黑莓籽石油醚部位、正丁醇部位及黑莓籽油具有一定程度的调节脂质代谢和抗脂质过氧化,改善脂质在小鼠肝脏和脾中的蓄积,减少氧化应激,推测其可能是黑莓籽有效部位降血脂的作用机制之一.

Abstract:

Objective: To study the effects of petroleum ether extract, n-butanol extract and Rubus spp seed oil from R. spp seed on lipid metabolism and lipid peroxidation of hyperlipidemia in mice. Method: One hundred and twenty mice were randomly divided into 12 groups:blank group, model group, positive control group, petroleum ether extract high dose group (800 mg·kg^-1), medium dose group (400 mg·kg^-1) and low dose group (200 mg·kg^-1), n-butanol extract high dose group (600 mg·kg^-1), medium dose group (300 mg·kg^-1) and low dose group (150 mg·kg^-1), R. spp seed oil high dose group (1 000 mg·kg^-1), medium dose group (500 mg·kg^-1) and low dose group (250 mg·kg^-1). The mice hyperlipidemia model was established by ig high fat emul, the contents of triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA),and superoxide dismutase(SOD)activity in serum, and the coefficients of liver, kidney and spleen were tested. Result: Low dose group of petroleum ether extract could significantly decrease the contents of TG, TC, LDL-C and MDA and the coefficients of liver and spleen, and increase the content of HDL-C and the activity of SOD (P<0.05). Medium and low dose groups of n-butanol extract could significantly decrease the contents of TG, TC, LDL-C and MDA and the coefficients of liver and spleen and increase the activities of SOD(P<0.05). Medium dose group of R. spp seed oil could significantly decrease the contents of TG, TC and MDA and the coefficients of liver and spleen and increase the content of HDL-C and the activity of SOD(P<0.05). High dose group of R. spp seed oil could significantly decrease the contents of TG, TC and MDA and the coefficients of liver and spleen, and increase the activity of SOD (P<0.05). High dose group of n-butanol extract could significantly decrease the contents of TG and MDA and the coefficients of liver and spleen and increase the content of HDL-C and the activity of SOD (P<0.05). Low dose group of R. spp seed oil could significantly decrease the contents of TG, TC, LDL-C and the coefficients of liver and spleen and increase the content of HDL-C (P<0.05). High (TG and TC) and medium (TC, HDL-C), liver and spleen coefficients dose groups of petroleum ether extract were significant change. Compared with model group, other indicators had no significance, but there was a certain effect. Conclusion: Petroleum ether extract, n-butanol extract and R. spp seed oil had a certain degree of regulating lipid metabolism and lipid peroxidation. They could improve lipid accumulation in the liver and spleen of mice. It might be one of the possible mechanism of effective extracts of R. spp seed for reducing blood lipid.