基于方证对应理论的黄连解毒汤肠道吸收分析

Intestinal Absorption Analysis of Huanglian Jiedu Tang Based on Theory of Correspondence Between Syndrome and Prescription

摘要:

目的: 基于方证对应理论研究正常和脑缺血病理状态下黄连解毒汤中指标成分肠吸收情况的差异性,探讨临床对症治疗脑缺血疾病的合理性。方法: 以黄连解毒汤中盐酸小檗碱、黄芩苷、栀子苷为检测指标,建立在体单向肠灌流模型,考察不同浓度黄连解毒汤在正常和脑缺血大鼠的肠吸收情况。结果: 与正常组比较,不同浓度黄连解毒汤中盐酸小檗碱、黄芩苷、栀子苷在脑缺血模型组大鼠不同肠段的肠吸收均有一定程度增加,其中盐酸小檗碱在十二指肠、空肠有显著性增加;黄芩苷在全部肠段的肠吸收均有显著性增加;栀子苷在空肠、回肠、结肠有显著性增加。结论: 黄连解毒汤主要药效成分在脑缺血大鼠的肠道吸收情况优于正常组,为该复方临床对症治疗脑缺血疾病的合理性提供实验依据,体现了中医方证对应理论。

Abstract:

Objective: To investigate intestinal absorption characteristics of index components from Huanglian Jiedu Tang (HLJDT) in normal and cerebral ischemia states,and discuss rationality of HLJDT used in clinical to treat cerebral ischemia,basing on theory of correspondence between syndrome and prescription. Method: Taking geniposide,berberine hydrochloride and baicalin in HLJDT as indexes,in situ single pass perfusion model was established to investigate intestinal absorption characteristics of HLJDT with different concentrations in rats with normal and cerebral ischemia. Result: Compared with the normal group,absorption of geniposide,berberine hydrochloride and baicalin from HLJDT with different concentrations in all model group increased to some extent in different intestine segments.Berberine hydrochloride increased significantly in duodenum and jejunum.Baicalin increased significantly in the whole intestine.Intestinal absorption of geniposide increased significantly in jejunum,ileum and colon. Conclusion: Absorption of main active ingredients of HLJDT in the cerebral ischemia model group is better than that in the normal group,which supports rationality of widely use of HLJDT in the clinical application to exerting therapeutic effect and confirms theory of correspondence between syndrome and prescription.