影响酶解去除薏苡仁淀粉过程的关键参数考察

Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis

摘要:

目的: 考察影响高温α-淀粉酶酶解去除薏苡仁中淀粉的关键参数,为薏苡仁总多糖的应用提供参考。方法: 以还原糖含量及碘液检试样品颜色变化作为定量及定性指标,在单因素试验基础上,通过正交试验考察酶解温度、酶用量、酶解时间、酶解pH对薏苡仁中淀粉酶解程度的影响。结果: 最佳酶解参数为酶用量600 U·g^-1,酶解温度75 ℃,酶解pH 6.0,酶解时间120 min;薏苡仁总多糖水提液中还原糖质量分数约25%,且碘试液检测不变色,薏苡仁总多糖纯度达76.63%,较未去除淀粉的薏苡仁总多糖纯度54.41%显著提高。结论: 高温α-淀粉酶可简单、快速的去除薏苡仁中淀粉,有利于高纯度薏苡仁总多糖的获得。

Abstract:

Objective: To provide a reference for application of total polysaccharides in Coicis Semen by investigating effect of key parameters on high temperature α-amylase enzymolysis for starch in Coicis Semen. Method: Take the content of reducing sugar as a quantitative indicator and color change after iodine solution testing as a qualitative indicator,based on single factor tests,orthogonal test was adopted to investigate effects of the amount of enzyme,hydrolysis temperature,hydrolysis pH and reaction time on amylohydrolysis. Result: Optimal parameters were as follows:the amount of enzyme 600 U·g^-1,enzymolysis temperature 75 ℃,enzymatic pH of 6.0,enzymolysis time of 120 min.The content of reducing sugar in extract of total polysaccharides in Coicis Semen was about 25%,meanwhile,color of samples did not alter after iodine solution testing.Purity of total polysaccharides in Coicis Semen was 76.63%,which showed a great improvement by comparing with 54.41% of samples without removing starch. Conclusion: High-temperature α-amylase can remove starch from Coicis Semen readily and rapidly,it is conducive to extraction of total polysaccharides.