壳聚糖絮凝法澄清天麻提取液的工艺优选

Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method

摘要:

目的: 优选壳聚糖澄清天麻提取液的工艺条件,为该药材的临床应用提供参考。方法: 采用HPLC测定天麻素含量,流动相乙腈-水(3:97),检测波长220 nm。以澄清效果和固形物含量为指标,在单因素试验基础上,通过正交试验考察壳聚糖添加量、药液浓缩比、絮凝温度、静置时间对天麻水提液壳聚糖絮凝工艺条件的影响。结果: 最佳工艺条件为壳聚糖添加量0.15%,药液浓缩比1:13 g·mL^-1,絮凝温度25 ℃,静置时间10 h。天麻素质量浓度65.93 g·L^-1,含固量3.157%。结论: 采用壳聚糖絮凝法澄清天麻提取液的方法简单易行、经济实用、澄清效果好,适用于中药水提液的精制。

Abstract:

Objective: To optimize clarification process of Gastrodiae Rhizoma extract with chitosan flocculating method. Method: HPLC was employed to determine the content of gastrodin with mobile phase of acetonitrile-water(3: 97) and detection wavelength at 220 nm.Taking clarification effect and solids content as indexes,on the basis of single factor tests,orthogonal design was used to optimize clarification process with the adding amount of chitosan,liquid concentration ratio,flocculation temperature and standing time as factors. Result: Optimal clarification procedure was obtained under these conditions:the adding amount of chitosan 0.15%,liquid concentration ratio 1.13 g·mL^-1,flocculation temperature at 25 ℃,standing time of 10 h.The concentration of gastrodin was 65.93 g·L^-1,solids content was 3.157%. Conclusion: This optimized process is feasible and economical with good clarification effect,which is suitable for purification of Chinese medicine extract.