响应面法优选N-14菌株发酵黄芪总多糖的工艺条件

Optimization of Strain N-14 Fermentation Process of Total Polysaccharides from Astragali Radix by Response Surface Methodology

摘要:

目的: 优化益生菌N-14发酵黄芪总多糖的工艺条件。方法: 采用UV测定总多糖含量,检测波长620 nm。在单因素试验基础上,以总多糖得率为响应值,底物添加量、发酵时间和灭菌时间为自变量,通过响应面试验优选黄芪总多糖发酵工艺。结果: 最佳发酵条件为底物添加量4 g,发酵时间24 h,灭菌时间15 min,pH 7,接种量1%。黄芪总多糖得率6.56%,与预测值6.69%偏差较小,较水提醇沉工艺的4.17%提高了57.31%。结论: 响应面分析法可用于优选益生菌N-14发酵黄芪的工艺条件,优选的工艺条件稳定可行,为该药材资源的充分利用提供参考。

Abstract:

Objective: To optimize fermentation conditions of total polysaccharides from Astragali Radix by probiotic N-14. Method: UV was adopted to determine the content of total polysaccharides with detection wavelength at 620 nm.Based on single factor tests,response surface methodology(RSM) was employed to optimize fermentation process of total polysaccharides from Astragali Radix with yield of total polysaccharides as response value and sterilization time,fermentation time,the amount of substrate as independent variables. Result: Optimum fermentation conditions were as follows:the amount of substrate 4 g,fermentation time of 24 h,sterilization time of 15 min,pH of 7,inoculation of probiotic 1%.Yield of total polysaccharides was up to 6.56%,which was lower than the predictive value of 6.69%,but 57.31% higher than yield of total polysaccharides(4.17%) by water extraction and alcohol precipitation process. Conclusion: RSM is suited to optimize fermentation process of Astragali Radix with probiotic N-14,this optimized process is stable and feasible,it provides a reference for utilization of this herb.