HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量

Simultaneous Determination of Five Compounds in Sanjie Zhentong Capsules by High-performance Liquid Chromatography Coupled with Ultraviolet and Evaporative Light Scattering Detectors

摘要:

目的: 建立HPLC-UV-ELSD同时测定散结镇痛胶囊中皂苷类和黄酮类成分的方法,比较15批散结镇痛胶囊中各成分含量。方法: 采用HPLC-UV-ELSD联用法,Waters Symmetry^@C₁₈色谱柱(4.6 mm×250 mm,5 μm),乙腈(A)-水(B)梯度洗脱(1~13 min,19%~37%A;13~50 min,37%~36%A),流速1 mL·min^-1,检测波长278 nm;蒸发光散射检测器(ELSD)漂移管温度45 ℃,载气流速3.7 L·min^-1,同时测定散结镇痛胶囊中三七皂苷R₁,人参皂苷Rg₁,人参皂苷Rb₁,龙血素A和龙血素B的含量。结果: 建立了同时测定散结镇痛胶囊中3个皂苷类和2个黄酮类成分含量的方法,线性关系良好(0.999 5~0.999 7),平均回收率为 99.1%~104.2%。结论: 该方法简便,重复性好,适用于散结镇痛胶囊皂苷类和黄酮类成分的同时测定,亦适用于散结镇痛胶囊的的质量控制。

Abstract:

Objective: To establish a quantitative method of three main saponins and two major active flavonoids in Sanjie Zhentong capsules by high-performance liquid chromatography coupled with ultraviolet detectors and evaporative light scattering detectors (HPLC-UV-ELSD), and compare the contents of notogin-senoside R₁, ginsenoside Rg₁,ginsenoside Rb₁,loureirin A, loureirin B among 15 batches of the drugs. Method: HPLC-UV-ELSD was conducted on a Waters Symmetry^@C₁₈(4.6 mm×250 mm, 5 μm) with gradient elution, the mobile phase was ACN and H₂O, the temperature of drift tube was 45 ℃ and the nebulizing gas flow rate was 3.7 L·min^-1. Result: Five compounds were simultaneously detected with one injection, good linear relationships were found(0.999 5-0.999 7), and the average recovery rates were 99.1%-104.2%. Conclusion: The HPLC-UV-ELSD method is simple and reproducible, which can be used for quality control of Sanjie Zhentong capsules.