辐射灭菌对黄芩中化学成分的影响

Influence of Irradiation Sterilization on Chemical Constituents of Scutellariae Radix

摘要:

目的: 用高效液相色谱法对经辐照灭菌的黄芩药材、黄芩药材粉末及未经灭菌黄芩药材中黄芩苷的含量进行测定。比较灭菌前后黄芩药材及其粉末中黄芩苷含量变化情况。方法: 采用Accurasil-C₁₈色谱柱(4.6 mm×250 mm,5 μm);流动相甲醇-水-磷酸(47:53:0.2),检测波长280 nm,流速1.0 mL·min^-1,柱温30 ℃,进样量10 μL。结果: 黄芩苷在0.163 2~0.816 0 μg线性关系良好(r²= 0.999 8),平均回收率为99.44%,RSD 1.6%。灭菌前后饮片与粉末中黄芩苷含量均有显著性变化。结论: 以黄芩苷含量为指标考察,表明黄芩药材不适宜应用辐照灭菌法进行灭菌。

Abstract:

Objective: HPLC was adopted to determine the baicalin content of the Scutellariae Radix and Scutellariae Radix powder by using irradiation sterilization, together with herbs without the sterilization. Compared the content changes before and after mirradiation sterilization. Method: Accurasil-C₁₈ chromatographic column(4.6 mm×250 mm,5 μm), methanol-water-phosphoric acid (47: 53: 0.2) as mobile phase, the wavelength detection was 280 nm with flow rate of 1.0 mL·min^-1, column temperature 30 ℃, and the injection volume of 10 μL. Result: Baicalin had good linear relationship in the range of 0.163 2-0.816 0 μg. The average recovery was 99.44% with the RSD of 1.6%. Content baicalin in Radix had significant changes before and after sterilization. Conclusion: It indicates that Scutellariae Radix is not suitable for applications of irradiation sterilization,which takes baicalin as index.