不同产地天麻药材薄层色谱指纹图谱分析

Fingerprint Analysis for TLC of Gastrodiae Rhizoma from Different Habitats

摘要:

目的: 建立天麻药材TLC指纹图谱的鉴别方法,比较不同产地天麻药材的指纹图谱。方法: 利用TLC建立天麻药材的鉴别方法,硅胶GF₂₅₄薄层板,加甲醇预展开,挥干甲醇,110 ℃活化30 min,以三氯甲烷-乙酸乙酯-甲醇-水(2.5:1:1:0.1) 为展开剂,展开环境为温度20~25 ℃,相对湿度60%~70%,喷以10%硫酸乙醇溶液于105 ℃显色,置紫外光灯(366 nm)下检识并成像,利用指纹图谱系统解决方案软件生成共有模式。通过聚类分析和主成分分析30批天麻药材的差异性。结果: 建立的天麻TLC斑点显色清晰。30批天麻药材聚类分析可分为5类,主成分分析分为6类,可区分不同产地的天麻药材。结论: 不同地理居群、环境气候的天麻样品可能会因地理、空间距离和温度、湿度差异造成多样性分化,在化学成分含量方面呈现出一定差异。建立的TLC指纹图谱可用于控制不同来源天麻药材的质量。

Abstract:

Objective: To establish a TLC fingerprint for identifying Gastrodiae Rhizoma and compare fingerprint of Gastrodiae Rhizoma from different habitats. Method: A TLC method for identification of Gastrodiae Rhizoma was established with silica gel GF₂₅₄ TLC plate and adding methanol for pre-deployment,methanol was evaporated to dryness,activation for 30 min at 110 ℃,with chloroform-ethyl acetate-methanol-water(2.5: 1: 1: 0.1) as developing agent,deployment temperature was 20-25 ℃,relative humidity was 60%-70%,sprayed with 10% sulfuric acid in ethanol at 105 ℃,viewing and imaging under UV light(366 nm),fingerprint software generated a total system solution model.Differences of 30 batches of Gastrodiae Rhizoma were investigated through cluster analysis and principal component analysis. Result: This established TLC had a clear spot color.Cluster analysis of thirty batches of Gastrodiae Rhizoma was divided into five categories,principal component analysis was divided into six categories,it could distinguish Gastrodiae Rhizoma from different habitats. Conclusion: Diversity differentiation of Gastrodiae Rhizoma may be due to geography,spatial distance,temperature and humidity,in terms of chemical compositions shows some differences.This established TLC fingerprint can be used to control quality of Gastrodiae Rhizoma from different sources.